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Literature summary for 4.1.2.55 extracted from

  • Wolterink-van Loo, S.; Siemerink, M.A.; Perrakis, G.; Kaper, T.; Kengen, S.W.; van der Oost, J.
    Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase (2009), Archaea, 2, 233-239.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.55

Protein Variants

Protein Variants Comment Organism
V193A the mutant enzyme displays a threefold increase in activity compared with wild type enzyme. Increased specific activity at 40–60 °C of this mutant is observed, not only for the condensation of pyruvate with glyceraldehyde, but also for several unnatural acceptor aldehydes. The optimal temperature for activity of SacKdgAV193A is lower than for the wild type enzyme, but enzymatic stability of the mutant is similar to that of the wild type Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 pyruvate pH 6.0, 50°C, wild-type enzyme Sulfolobus acidocaldarius
2.1
-
 D-glyceraldehyde pH 6.0, 50°C, wild-type enzyme Sulfolobus acidocaldarius
5.4
-
 pyruvate pH 6.0, 50°C, mutant enzyme V193A Sulfolobus acidocaldarius
5.4
-
 D-glyceraldehyde pH 6.0, 50°C, mutant enzyme V193A Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JC35
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + 2-azidoacetaldehyde
-
 Sulfolobus acidocaldarius ?
-
 ?
pyruvate + 3-azido-2-hydroxypropanal
-
 Sulfolobus acidocaldarius ?
-
 ?
pyruvate + D-glyceraldehyde
-
 Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-galactonate
-
 ?
pyruvate + D-glyceraldehyde
-
 Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-gluconate
-
 ?
pyruvate + glycolaldehyde
-
 Sulfolobus acidocaldarius ?
-
 ?

Synonyms

Synonyms Comment Organism
2-keto-3-deoxygluconate aldolase
-
 Sulfolobus acidocaldarius
SacKdgA
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 mutant enzyme V193A Sulfolobus acidocaldarius
90
-
 wild-type enzyme Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the mutant enzyme V193A is more active than wild-type Sulfolobus acidocaldarius
50 90 50°C: about 65% of maximal activity, 90°C: about 70% of maximal activity, mutant enzyme V193A Sulfolobus acidocaldarius
60 90 60°C: about 50% of maximal activity, 90°C: about 95% of maximal activity, wild-type enzyme Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 activity half-life of wild-type enzyme is 100 min, activity half-life of mutant enzyme V193A is 120 min Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 D-glyceraldehyde pH 6.0, 50°C, wild-type enzyme Sulfolobus acidocaldarius
5.5
-
 pyruvate pH 6.0, 50°C, wild-type enzyme Sulfolobus acidocaldarius
15
-
 pyruvate pH 6.0, 50°C, mutant enzyme V193A Sulfolobus acidocaldarius
15.7
-
 D-glyceraldehyde pH 6.0, 50°C, mutant enzyme V193A Sulfolobus acidocaldarius