Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.53 extracted from

  • Rakus, J.F.; Fedorov, A.A.; Fedorov, E.V.; Glasner, M.E.; Hubbard, B.K.; Delli, J.D.; Babbitt, P.C.; Almo, S.C.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: L-rhamnonate dehydratase (2008), Biochemistry, 47, 9944-9954.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C Escherichia coli
0.14
-
 2-dehydro-3-deoxy-L-mannonate pH 8.0, 25°C Escherichia coli
0.8
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76469
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-lyxonate
-
 Escherichia coli ?
-
 ?
2-dehydro-3-deoxy-L-mannonate
-
 Escherichia coli ?
-
 ?
2-dehydro-3-deoxy-L-rhamnonate 2-dehydro-3-deoxy-L-rhamnonate i.e. 3,6-dideoxy-L-erythro-hex-2-ulosonate Escherichia coli pyruvate + (R)-lactaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
2-dehydro-3-deoxyrhamnonate aldolase
-
 Escherichia coli
2-keto-3-deoxy acid sugar aldolase
-
 Escherichia coli
KDR aldolase
-
 Escherichia coli
YfaU
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C Escherichia coli
0.3
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C Escherichia coli
0.4
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 2-dehydro-3-deoxy-L-mannonate pH 8.0, 25°C Escherichia coli
3.8
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C Escherichia coli
5.1
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C Escherichia coli