Cloned (Comment) | Organism |
---|---|
gene queD, overexpression in Escherichia coli strain BL21 (DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified recomb inant QueD, hanging-drop vapor-diffusion method, mixing of 800 nl of protein solution containing 10 mg/ml protein and of 800 nl crystallization solution containing 25% PEG 600, 100 mM NaCl and sodium citrate buffer, pH 5.5, at 18°C, X-ray diffraction structure determination and analysis at 3.6 A resolution | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene queD | - |
Purification (Comment) | Organism |
---|---|
recombinant QueD from Escherichia coli strain BL21 (DE3) by anion exchange and hydrophobic inetraction chromatography | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
QueD | formerly YkvK | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | QueD is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Formation of queuosine is a multistep reaction that can be divided into three stages, overview | Bacillus subtilis |