Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
DL-threo-phenylserine | pH 8.0, 30°C | Escherichia coli | |
0.22 | - |
L-allo-threonine | pH 8.0, 30°C | Escherichia coli | |
0.24 | - |
DL-erythro-phenylserine | pH 8.0, 30°C | Escherichia coli | |
2.85 | - |
L-threonine | pH 8.0, 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
4 * 36500, SDS-PAGE | Escherichia coli |
140000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75823 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
pH 8.0, 30°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-erythro-phenylserine | - |
Escherichia coli | glycine + benzaldehyde | - |
? | |
DL-threo-phenylserine | - |
Escherichia coli | glycine + benzaldehyde | - |
? | |
L-allo-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
r | |
L-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36500, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
LtaE | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
65 | 70 | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, stable | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8.5 | 9 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | 30°C, 30 min, stable | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | contains 1 mol of pyridoxal 5'-phosphate as cofactor per mol of 36500 Da subunit | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | knockout of the ltaE gene of wild-type Escherichia coli does not affect the cellular growth rate, while disruption of the ltaE gene of Escherichia coli GS245, whose serine hydroxymethyltransferase gene is knocked out, causes a significant decrease in the cellular growth rate, suggesting that the threonine aldolase is not a major source of cellular glycine in wild-type Escherichia coli but catalyzes an alternative pathway for cellular glycine when serine hydroxymethyltransferase is inert | Escherichia coli |
physiological function | threonine aldolase is not a major source of cellular glycine in wild-type Escherichia coli but catalyzes an alternative pathway for cellular glycine when serine hydroxymethyltransferase is inert | Escherichia coli |