Any feedback?
Literature summary for 4.1.2.48 extracted from
- Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli (1998), Eur. J. Biochem., 255, 220-226.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
DL-threo-phenylserine | pH 8.0, 30°C | Escherichia coli |  |
| 0.22 | - |
L-allo-threonine | pH 8.0, 30°C | Escherichia coli | |
| 0.24 | - |
DL-erythro-phenylserine | pH 8.0, 30°C | Escherichia coli | |
| 2.85 | - |
L-threonine | pH 8.0, 30°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
4 * 36500, SDS-PAGE | Escherichia coli |
| 140000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P75823 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.7 | - |
pH 8.0, 30°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-erythro-phenylserine | - |
Escherichia coli | glycine + benzaldehyde | - |
? | |
| DL-threo-phenylserine | - |
Escherichia coli | glycine + benzaldehyde | - |
? | |
| L-allo-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
r | |
| L-threonine | - |
Escherichia coli | glycine + acetaldehyde | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36500, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LtaE | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 65 | 70 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, stable | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
| 8.5 | 9 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | 30°C, 30 min, stable | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | contains 1 mol of pyridoxal 5'-phosphate as cofactor per mol of 36500 Da subunit | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | knockout of the ltaE gene of wild-type Escherichia coli does not affect the cellular growth rate, while disruption of the ltaE gene of Escherichia coli GS245, whose serine hydroxymethyltransferase gene is knocked out, causes a significant decrease in the cellular growth rate, suggesting that the threonine aldolase is not a major source of cellular glycine in wild-type Escherichia coli but catalyzes an alternative pathway for cellular glycine when serine hydroxymethyltransferase is inert | Escherichia coli |
| physiological function | threonine aldolase is not a major source of cellular glycine in wild-type Escherichia coli but catalyzes an alternative pathway for cellular glycine when serine hydroxymethyltransferase is inert | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
