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Literature summary for 4.1.2.47 extracted from
- Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters (2014), ChemBioChem, 15, 1931-1938 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Hevea brasiliensis |
| expression of wild-type and mutant enzymes in Escherichia coli BL21 | Hevea brasiliensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C81L | 1.3fold increase in kcat for racemic mandelonitrile | Hevea brasiliensis |
| C81L | the mutant has high enantioselectivity towards (R)-mandelonitrile with a kcat value 1.3times higher compared to the wild type enzyme | Hevea brasiliensis |
| F125T | 2.6fold increase in kcat for racemic mandelonitrile, 4.5fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| F125T | the mutant has high enantioselectivity towards (R)-mandelonitrile with a kcat value 2.6times and a kcat/Km value 4.5times higher compared to the wild type enzyme | Hevea brasiliensis |
| F125T/L146M | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| F125T/Y133F | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| F210I | mutant yields mostly insoluble protein, which suggests that the substitution hinders protein folding | Hevea brasiliensis |
| F210I | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| I12A | mutant yields mostly insoluble protein, which suggests that the substitution hinders protein folding | Hevea brasiliensis |
| I209A | 3.9fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| I209A | the mutant has higher enantioselectivity towards (R)-mandelonitrile with a kcat/Km value 3.9times higher compared to the wild type enzyme | Hevea brasiliensis |
| I209G | mutant yields mostly insoluble protein, which suggests that the substitution hinders protein folding, moderate substrate inhibition by rac-mandelonitrile | Hevea brasiliensis |
| I209G | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L121F | 6.9fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| L121Y | 5.3fold increase in specificity constants (kcat/Km) for racemic mandelonitrile, 4.2fold increase in kcat for racemic mandelonitrile, the mutant enzyme shows the same high (S)-enantioselectivity (98% ee) as wild-type enzyme. The mutant catalyzes the cleavage of 2-nitro-1-phenylethanol 4.8 times more efficiently than the wild type enzyme, this is similar to the 4.5-fold increase seen for mandelonitrile cleavage | Hevea brasiliensis |
| L121Y | the mutant has high enantioselectivity towards (S)-mandelonitrile with a kcat value 4.2times higher and a kcat/Km value 5.3times higher compared to the wild type enzyme | Hevea brasiliensis |
| L121Y/F125T | 1.7fold increase in kcat for racemic mandelonitrile, 1.9fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| L121Y/F125T | the mutant has high enantioselectivity towards (R)-mandelonitrile with a kcat value 4.2times higher and a kcat/Km value 1.7times higher compared to the wild type enzyme | Hevea brasiliensis |
| L121Y/F125T | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L121Y/F125T/L146M | 5.5fold increase in activity over the wild type enzyme | Hevea brasiliensis |
| L121Y/F125T/L146M | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L121Y/L146M | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L146M | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L148F | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| L152F | 1.5fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| L152F | the mutant has higher enantioselectivity towards (R)-mandelonitrile with a kcat/Km value 1.5times higher compared to the wild type enzyme | Hevea brasiliensis |
| V106F | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| V106F/L121Y | the mutant shows higher specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
| V106F/L121Y/F125T | 3.3fold increase in kcat for racemic mandelonitrile, 5.5fold increase in specificity constants (kcat/Km) for racemic mandelonitrile | Hevea brasiliensis |
| V106F/L121Y/F125T | the mutant has high enantioselectivity towards (R)-mandelonitrile with a kcat value 3.3times higher compared to the wild type enzyme | Hevea brasiliensis |
| Y133F | the mutant shows lower specific activity towards racemic mandelonitrile compared to the wild type enzyme | Hevea brasiliensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis |  |
| 0.4 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis | |
| 0.6 | - |
mandelonitrile | mutant enzyme L152F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L152F | Hevea brasiliensis | |
| 0.7 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.7 | - |
mandelonitrile | mutant enzyme I209G, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.7 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis | |
| 0.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209G | Hevea brasiliensis | |
| 0.8 | - |
mandelonitrile | mutant enzyme I209A, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.8 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209A | Hevea brasiliensis | |
| 1.4 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.4 | - |
mandelonitrile | mutant enzyme V106F/L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.4 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis | |
| 1.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y | Hevea brasiliensis | |
| 1.5 | - |
mandelonitrile | mutant enzyme F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis | |
| 1.5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121F | Hevea brasiliensis | |
| 1.6 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.6 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y | Hevea brasiliensis | |
| 1.8 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.8 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T | Hevea brasiliensis | |
| 2 | - |
mandelonitrile | mutant enzyme F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 2 | - |
mandelonitrile | mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 2 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis | |
| 2 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T | Hevea brasiliensis | |
| 2.1 | - |
mandelonitrile | mutant enzyme F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 2.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis | |
| 2.7 | - |
mandelonitrile | mutant enzyme L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 2.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121T | Hevea brasiliensis | |
| 2.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y | Hevea brasiliensis | |
| 2.8 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121A | Hevea brasiliensis | |
| 3.1 | - |
mandelonitrile | mutant enzyme L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.1 | - |
mandelonitrile | mutant enzyme Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T | Hevea brasiliensis | |
| 3.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis | |
| 3.3 | - |
mandelonitrile | wild type enzyme, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.3 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis | |
| 3.6 | - |
mandelonitrile | mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M | Hevea brasiliensis | |
| 3.7 | - |
mandelonitrile | mutant enzyme L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121V | Hevea brasiliensis | |
| 3.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L146M | Hevea brasiliensis | |
| 4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121M | Hevea brasiliensis | |
| 4.9 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121I | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme C81L, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5.3 | - |
mandelonitrile | mutant enzyme V106F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5.3 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis | |
| 5.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121W | Hevea brasiliensis | |
| 5.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Q | Hevea brasiliensis | |
| 6.1 | - |
2-nitro-1-phenylethanol | wild type enzyme, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 6.1 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis | |
| 6.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121S | Hevea brasiliensis | |
| 6.7 | - |
mandelonitrile | mutant enzyme L121Y/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 6.7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M | Hevea brasiliensis | |
| 7.1 | - |
2-nitro-1-phenylethanol | mutant enzyme Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 7.1 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis | |
| 8 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121H | Hevea brasiliensis | |
| 8.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121C | Hevea brasiliensis | |
| 8.6 | - |
mandelonitrile | mutant enzyme F210I, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 8.6 | - |
mandelonitrile | mutant enzyme L148F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 8.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F210I | Hevea brasiliensis | |
| 8.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L148F | Hevea brasiliensis | |
| 9.4 | - |
mandelonitrile | mutant enzyme C81L, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 9.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme C81L | Hevea brasiliensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-2-methylpropanenitrile | Hevea brasiliensis | - |
cyanide + propan-2-one | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hevea brasiliensis | P52704 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hevea brasiliensis |
| Ni-NTA agarose column chromatography and Sephadex G-75 gel filtration | Hevea brasiliensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121P | Hevea brasiliensis |
| 0.13 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M | Hevea brasiliensis |
| 0.13 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis |
| 0.15 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme C81L | Hevea brasiliensis |
| 0.17 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis |
| 0.2 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis |
| 0.2 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121E | Hevea brasiliensis |
| 0.21 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis |
| 0.21 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L146M | Hevea brasiliensis |
| 0.27 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis |
| 0.29 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T | Hevea brasiliensis |
| 0.29 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis |
| 0.32 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L146M | Hevea brasiliensis |
| 0.39 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T | Hevea brasiliensis |
| 0.56 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/Y133F | Hevea brasiliensis |
| 0.62 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y | Hevea brasiliensis |
| 0.7 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121N | Hevea brasiliensis |
| 0.71 | - |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M | Hevea brasiliensis |
| 0.9 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme I12A | Hevea brasiliensis |
| 1.4 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121G | Hevea brasiliensis |
| 2 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121T | Hevea brasiliensis |
| 3.8 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121S | Hevea brasiliensis |
| 4 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121H | Hevea brasiliensis |
| 5.1 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme I209G | Hevea brasiliensis |
| 5.9 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121Q | Hevea brasiliensis |
| 6.7 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis |
| 7 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L148F | Hevea brasiliensis |
| 12 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L152F | Hevea brasiliensis |
| 14 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme F210I | Hevea brasiliensis |
| 14 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121W | Hevea brasiliensis |
| 16 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121C | Hevea brasiliensis |
| 16 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121V | Hevea brasiliensis |
| 22 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme C81L | Hevea brasiliensis |
| 24 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L146M | Hevea brasiliensis |
| 28 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis |
| 30 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121A | Hevea brasiliensis |
| 33 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis |
| 35 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121I | Hevea brasiliensis |
| 42 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme I209A | Hevea brasiliensis |
| 52 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis |
| 52 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121M | Hevea brasiliensis |
| 53 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis |
| 55 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T | Hevea brasiliensis |
| 64 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M | Hevea brasiliensis |
| 88 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y | Hevea brasiliensis |
| 92 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis |
| 115 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M | Hevea brasiliensis |
| 119 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121F | Hevea brasiliensis |
| 140 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T | Hevea brasiliensis |
| 149 | - |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y | Hevea brasiliensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-2-methylpropanenitrile | - |
Hevea brasiliensis | cyanide + propan-2-one | - |
r | |
| 2-nitro-1-phenylethanol | - |
Hevea brasiliensis | nitromethane + benzaldehyde | - |
? | |
| benzaldehyde + HCN | - |
Hevea brasiliensis | (S)-mandelonitrile | - |
? | |
| mandelonitrile | - |
Hevea brasiliensis | cyanide + benzaldehyde | - |
? | |
| rac-2-nitro-1-phenylethanol | - |
Hevea brasiliensis | nitromethane + benzaldehyde | - |
? | |
| rac-mandelonitrile | - |
Hevea brasiliensis | benzaldehyde + HCN | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HNL | - |
Hevea brasiliensis |
| Hydroxynitrile lyase | - |
Hevea brasiliensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.1 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.1 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.1 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.1 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis | |
| 0.1 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis | |
| 0.12 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.12 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis | |
| 0.15 | - |
2-nitro-1-phenylethanol | wild type enzyme, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.15 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis | |
| 0.2 | - |
2-nitro-1-phenylethanol | mutant enzyme C81L, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.2 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.2 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.2 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/F125T | Hevea brasiliensis | |
| 0.3 | - |
2-nitro-1-phenylethanol | mutant enzyme Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.3 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis | |
| 0.5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.5 | - |
2-nitro-1-phenylethanol | mutant enzyme L121Y/F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.5 | - |
2-nitro-1-phenylethanol | mutant enzyme L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.5 | - |
2-nitro-1-phenylethanol | mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.6 | - |
2-nitro-1-phenylethanol | mutant enzyme F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 0.6 | - |
rac-2-nitro-1-phenylethanol | pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis | |
| 3 | - |
mandelonitrile | mutant enzyme I209G, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209G | Hevea brasiliensis | |
| 3 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121T | Hevea brasiliensis | |
| 5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121H | Hevea brasiliensis | |
| 6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Q | Hevea brasiliensis | |
| 7 | - |
mandelonitrile | mutant enzyme L152F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 7 | - |
mandelonitrile | mutant enzyme V106F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L152F | Hevea brasiliensis | |
| 7 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis | |
| 9 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121S | Hevea brasiliensis | |
| 15 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121V | Hevea brasiliensis | |
| 15 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121W | Hevea brasiliensis | |
| 17 | - |
mandelonitrile | mutant enzyme L148F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 17 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L148F | Hevea brasiliensis | |
| 20 | - |
mandelonitrile | mutant enzyme L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 20 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121C | Hevea brasiliensis | |
| 20 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L146M | Hevea brasiliensis | |
| 21 | - |
mandelonitrile | mutant enzyme F210I, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 21 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F210I | Hevea brasiliensis | |
| 22 | - |
mandelonitrile | mutant enzyme Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 22 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis | |
| 23 | - |
mandelonitrile | mutant enzyme I209A, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 23 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209A | Hevea brasiliensis | |
| 23 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121A | Hevea brasiliensis | |
| 25 | - |
mandelonitrile | wild type enzyme, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 25 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis | |
| 32 | - |
mandelonitrile | mutant enzyme F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 32 | - |
mandelonitrile | mutant enzyme F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 32 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis | |
| 32 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis | |
| 33 | - |
mandelonitrile | mutant enzyme C81L, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 33 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme C81L | Hevea brasiliensis | |
| 33 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121I | Hevea brasiliensis | |
| 43 | - |
mandelonitrile | mutant enzyme L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 43 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121M | Hevea brasiliensis | |
| 43 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T | Hevea brasiliensis | |
| 45 | - |
mandelonitrile | mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 45 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M | Hevea brasiliensis | |
| 55 | - |
mandelonitrile | mutant enzyme V106F/L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 55 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y | Hevea brasiliensis | |
| 66 | - |
mandelonitrile | mutant enzyme F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 66 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis | |
| 77 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121F | Hevea brasiliensis | |
| 82 | - |
mandelonitrile | mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 82 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T | Hevea brasiliensis | |
| 106 | - |
mandelonitrile | mutant enzyme L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 106 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y | Hevea brasiliensis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121H | Hevea brasiliensis | |
| 1 | - |
mandelonitrile | mutant enzyme L148F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L148F | Hevea brasiliensis | |
| 1.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Q | Hevea brasiliensis | |
| 1.4 | - |
mandelonitrile | mutant enzyme V106F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 1.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121S | Hevea brasiliensis | |
| 1.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121T | Hevea brasiliensis | |
| 1.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F | Hevea brasiliensis | |
| 2.5 | - |
mandelonitrile | mutant enzyme F210I, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 2.5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F210I | Hevea brasiliensis | |
| 2.5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121C | Hevea brasiliensis | |
| 2.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121W | Hevea brasiliensis | |
| 3.5 | - |
mandelonitrile | mutant enzyme C81L, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 3.5 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme C81L | Hevea brasiliensis | |
| 4 | - |
mandelonitrile | mutant enzyme I209G, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209G | Hevea brasiliensis | |
| 4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121V | Hevea brasiliensis | |
| 5.4 | - |
mandelonitrile | mutant enzyme L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 5.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L146M | Hevea brasiliensis | |
| 6.6 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121I | Hevea brasiliensis | |
| 7.1 | - |
mandelonitrile | mutant enzyme Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 7.1 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme Y133F | Hevea brasiliensis | |
| 7.4 | - |
mandelonitrile | wild type enzyme, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 7.4 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, wild-type enzyme | Hevea brasiliensis | |
| 8.3 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121A | Hevea brasiliensis | |
| 11 | - |
mandelonitrile | mutant enzyme L152F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 11 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121M | Hevea brasiliensis | |
| 11 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L152F | Hevea brasiliensis | |
| 12 | - |
mandelonitrile | mutant enzyme L121Y/F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 12 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T/L146M | Hevea brasiliensis | |
| 14 | - |
mandelonitrile | mutant enzyme L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 14 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/F125T | Hevea brasiliensis | |
| 15 | - |
mandelonitrile | mutant enzyme F125T/Y133F, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 15 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/Y133F | Hevea brasiliensis | |
| 21 | - |
mandelonitrile | mutant enzyme F125T/L146M, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 21 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M | Hevea brasiliensis | |
| 29 | - |
mandelonitrile | mutant enzyme I209A, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 29 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme I209A | Hevea brasiliensis | |
| 33 | - |
mandelonitrile | mutant enzyme F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 33 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme F125T | Hevea brasiliensis | |
| 39 | - |
mandelonitrile | mutant enzyme L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 39 | - |
mandelonitrile | mutant enzyme V106F/L121Y, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 39 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121Y | Hevea brasiliensis | |
| 39 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y | Hevea brasiliensis | |
| 41 | - |
mandelonitrile | mutant enzyme V106F/L121Y/F125T, pH and temperature not specified in the publication | Hevea brasiliensis | |
| 41 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme V106F/L121Y/F125T | Hevea brasiliensis | |
| 51 | - |
rac-mandelonitrile | pH 5.0, temperature not specified in the publication, mutant enzyme L121F | Hevea brasiliensis | |
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