KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Hevea brasiliensis | |
1.4 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Manihot esculenta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | - |
- |
- |
Manihot esculenta | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelonitrile | - |
Hevea brasiliensis | cyanide + benzaldehyde | - |
? | |
(S)-mandelonitrile | - |
Manihot esculenta | cyanide + benzaldehyde | - |
r | |
cyanide + benzaldehyde | - |
Manihot esculenta | (S)-mandelonitrile | low enantioselectivity with 20% ee (S) | r |
Synonyms | Comment | Organism |
---|---|---|
(S)-selective hydroxynitrile lyase | - |
Manihot esculenta |
HNL | - |
Hevea brasiliensis |
HNL | - |
Manihot esculenta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Hevea brasiliensis | |
21.67 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Manihot esculenta |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.55 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Hevea brasiliensis | |
15.83 | - |
(S)-mandelonitrile | in 50 mM citrate buffer (pH 5.0), temperature not specified in the publication | Manihot esculenta |