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Literature summary for 4.1.2.47 extracted from
- Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4-oxocyclohexyl)acetonitrile as the model (2008), Chemistry, 14, 11415-11422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Hevea brasiliensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H103L/W128A | increased activity with the substrate (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile compared to the starting clone W128A | Hevea brasiliensis |
| W128A | higher conversion and better selectivity than wild-type enzyme with the substrate 4-methoxycyclohex-3-ene-1-carbaldehyde | Hevea brasiliensis |
| W128A | lower conversion and lower selectivity than wild-type enzyme with the substrate 4-methoxycyclohex-3-ene-1-carbaldehyde | Hevea brasiliensis |
| W128A/I219V | very weak activity in cleavage reaction with (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile | Hevea brasiliensis |
| W128A/K147R | very weak activity in cleavage reaction with (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile | Hevea brasiliensis |
| W128A/P187L | no activity in cleavage reaction with (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile | Hevea brasiliensis |
| W128A/Q215H | slightly increased activity with the substrate (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile compared to the starting clone W128A | Hevea brasiliensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hevea brasiliensis | P52704 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanide + 4-methoxycyclohex-3-ene-1-carbaldehyde | - |
Hevea brasiliensis | (2S)-hydroxy[4-(methoxy)cyclohex-3-en-1-yl]ethanenitrile | - |
r |  |
| cyanide + 4-oxocyclohexanecarbaldehyde | - |
Hevea brasiliensis | (2S)-hydroxy(4-oxocyclohexyl)ethanenitrile | - |
r | |
| cyanide + 4-[(trimethylsilyl)oxy]cyclohex-3-ene-1-carbaldehyde | - |
Hevea brasiliensis | (2S)-hydroxy[4-((trimethylsilyl)oxy)cyclohex-3-en-1-yl]ethanenitrile | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HbHNL | - |
Hevea brasiliensis |
| Hydroxynitrile lyase | - |
Hevea brasiliensis |
| Oxynitrilase | - |
Hevea brasiliensis |
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