Literature summary for 4.1.2.47 extracted from

Cabirol, F.L.; Hanefeld, U.; Sheldon, R.A.
Immobilized hydroxynitrile lyases for enantioselective synthesis of cyanohydrins: sol-gels and cross-linked enzyme aggregates (2006), Adv. Synth. Catal., 348, 1645-1654.

No PubMed abstract available

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Organism

Organism	UniProt	Comment	Textmining
Hevea brasiliensis	-	precipitated hydroxynitrile lyase, comparison of free, cross-linked enzyme aggregate (CLEA) and immobilized in sol-gel (aqua gel)	-

Purification (Commentary)

Purification (Comment)	Organism
preparation of a cross-linked enzyme aggregate (CLEA) from hydroxynitrile lyase from Hevea brasiliensis, precipitated hydroxynitrile lyase, comparison of free, cross-linked enzyme aggregate (CLEA) and immobilized in sol-gel (aqua gel)	Hevea brasiliensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-furaldehyde cyanohydrin	aqua gel, 30 min, conversion ratio: 89%, enantiomeric excess: 94%	Hevea brasiliensis	2-furaldehyde + HCN	-	r
2-furaldehyde cyanohydrin	free enzyme, 30 min, conversion ratio: 89%,enantiomeric excess: 94%	Hevea brasiliensis	2-furaldehyde + HCN	-	r
hexanal cyanohydrin	aqua gel, 2 h, conversion ratio: 92%, enantiomeric excess: 94%	Hevea brasiliensis	hexanal + HCN	-	r
hexanal cyanohydrin	free enzyme, 4 h, conversion ratio: 91%,enantiomeric excess: 94%	Hevea brasiliensis	hexanal + HCN	-	r
m-phenoxybenzaldehyde cyanohydrin	aqua gel, 72 h, conversion ratio: 92%, enantiomeric excess: 98%	Hevea brasiliensis	m-phenoxybenzaldehyde + HCN	-	r
m-phenoxybenzaldehyde cyanohydrin	free enzyme, 72 h, conversion ratio: 45%,enantiomeric excess: 82%	Hevea brasiliensis	m-phenoxybenzaldehyde + HCN	-	r
mandelonitrile	aqua gel, 0.5 h, conversion ratio: 97%, enantiomeric excess: 99%	Hevea brasiliensis	benzaldehyde + HCN	-	r
mandelonitrile	CLEA, 72 h, conversion ratio: 55%, enantiomeric excess: 67%	Hevea brasiliensis	benzaldehyde + HCN	-	r
mandelonitrile	free enzyme, 4 h, conversion ratio: 97%,enantiomeric excess: 97%	Hevea brasiliensis	benzaldehyde + HCN	-	r

Synonyms

Synonyms	Comment	Organism
(S)-HbHNL	-	Hevea brasiliensis
HbHNL	-	Hevea brasiliensis
Hydroxynitrile lyase	-	Hevea brasiliensis
Oxynitrilase	-	Hevea brasiliensis

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Release 2023.1 (January 2023)