Crystallization (Comment) | Organism |
---|---|
vapor diffusion hanging drop method, crystal structure of the mutant enzyme S80A refined to an R-factor of 18.0% against diffraction data to 2.1 A resolution, determination of three-dimensional structure of the complex of mutant enzyme S80A with acetone cyanohydrin at 2.2 A resultion, refined to an R-factor of 18.7% | Manihot esculenta |
Protein Variants | Comment | Organism |
---|---|---|
C81A | specific activity is 93% of the wild-type value | Manihot esculenta |
T11A | specific activity is 24fold lower than that of the wild-type enzyme | Manihot esculenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
169 | - |
acetone cyanohydrin | mutant enzyme C81A | Manihot esculenta | |
174 | - |
acetone cyanohydrin | wild-type enzyme | Manihot esculenta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manihot esculenta | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-2-methylpropanenitrile | - |
Manihot esculenta | HCN + acetone | - |
? |