Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not sensitive to 5 mM EDTA | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q51947 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.55 | - |
recombinant enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2'-hydroxybenzylidenepyruvate + O2 | the equilibrium in this reaction favors cleavage | Pseudomonas putida | salicylaldehyde + pyruvate | - |
r | |
additional information | no activity with trans-benzylidenepyruvate, trans-o-methoxybenzylidenepyruvate or trans-o-hydroxycinnamate. The hydratase-aldolase catalyzes the condensation of pyruvate with several other aromatic aldehydes, including benzaldehyde, to give trans-benzylidenepyruvate. Since benzylidenepyruvate is not a substrate for the enzyme, the reaction is irreversible and can be carried out to completion by using relatively low concentrations of the substrates, benzaldehyde and pyruvate | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tHBPA hydratase-aldolase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Pseudomonas putida |