Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the enzyme is completely inhibited by the presence of 2 mM EDTA | Mycobacterium gastri | |
additional information | the enzyme is inactivated irreversibly on dialysis against buffer without Mg2+ | Mycobacterium gastri | |
o-phenanthroline | the enzyme is completely inhibited by the presence of 2 mM o-phenanthroline | Mycobacterium gastri | |
Tiron | the enzyme is completely inhibited by the presence of 2 mM tiron | Mycobacterium gastri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
formaldehyde | in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C | Mycobacterium gastri | |
4.3 | - |
glycolaldehyde | in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C | Mycobacterium gastri | |
5.7 | - |
methylglyoxal | in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C | Mycobacterium gastri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential requirement for Mg2+ | Mycobacterium gastri | |
Mn2+ | essential requirement for Mn2+ | Mycobacterium gastri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
TSK gel G-3000SW chromatography | Mycobacterium gastri |
48000 | - |
2 * 48000, SDS-PAGE | Mycobacterium gastri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium gastri | - |
- |
- |
Mycobacterium gastri MB19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography, and DEAE-Sephacel column chromatography | Mycobacterium gastri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
cell free extract, at 30°C | Mycobacterium gastri |
74.2 | - |
after 23fold purification, at 30°C | Mycobacterium gastri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + formaldehyde | the enzyme is highly specific for D-ribulose 5-phosphate as an acceptor of aldehydes | Mycobacterium gastri | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + formaldehyde | the enzyme is highly specific for D-ribulose 5-phosphate as an acceptor of aldehydes | Mycobacterium gastri MB19 | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + glycolaldehyde | - |
Mycobacterium gastri | ? | - |
? | |
D-ribulose 5-phosphate + glycolaldehyde | - |
Mycobacterium gastri MB19 | ? | - |
? | |
D-ribulose 5-phosphate + methylglyoxal | - |
Mycobacterium gastri | ? | - |
? | |
D-ribulose 5-phosphate + methylglyoxal | - |
Mycobacterium gastri MB19 | ? | - |
? | |
additional information | no detectable activity with D-ribulose 1,5-bisphosphate, D-ribose 5-phosphate, D-erythrose 4-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, and D-xyluloSe 5-phosphate | Mycobacterium gastri | ? | - |
? | |
additional information | no detectable activity with D-ribulose 1,5-bisphosphate, D-ribose 5-phosphate, D-erythrose 4-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, and D-xyluloSe 5-phosphate | Mycobacterium gastri MB19 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48000, SDS-PAGE | Mycobacterium gastri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | remains stable in the pH range of 6.5-7.5 at 30°C for 6 h | Mycobacterium gastri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | in potassium phosphate buffer | Mycobacterium gastri |