Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 45% inhibition with 1 mM Cu2+ | Methylomonas aminofaciens | |
EDTA | the enzyme is completely inhibited in the presence of EDTA, but the activity is restored depending on the amount of MgC12 supplemented | Methylomonas aminofaciens | |
Glutaraldehyde | 22% inhibition at 4 mM | Methylomonas aminofaciens | |
glyceraldehyde | 13% inhibition at 4 mM | Methylomonas aminofaciens | |
glycolaldehyde | 49% inhibition at 4 mM | Methylomonas aminofaciens | |
glyoxylate | 12% inhibition at 4 mM | Methylomonas aminofaciens | |
Hg2+ | 81% inhibition with 1 mM Hg2+ | Methylomonas aminofaciens | |
methylglyoxal | 26% inhibition at 4 mM | Methylomonas aminofaciens | |
Pb2+ | 48% inhibition with 1 mM Pb2+ | Methylomonas aminofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
D-ribulose 5-phosphate | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens | |
0.29 | - |
formaldehyde | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the enzyme activity is also promoted with 1 mM Cd2+ | Methylomonas aminofaciens | |
Co2+ | the enzyme activity is also promoted with 5 mM Co2+ | Methylomonas aminofaciens | |
Fe2+ | the enzyme activity is also promoted with 1 mM Fe2+ | Methylomonas aminofaciens | |
Mg2+ | essential requirement for Mg2+ (5 mM) | Methylomonas aminofaciens | |
Mn2+ | essential requirement for Mn2+ (5 mM) | Methylomonas aminofaciens | |
Zn2+ | the enzyme activity is also promoted with 5 mM Zn2+ | Methylomonas aminofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
45000 | - |
native enzyme, gel filtration | Methylomonas aminofaciens |
47000 | - |
sedimentation velocity | Methylomonas aminofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylomonas aminofaciens | - |
- |
- |
Methylomonas aminofaciens 77a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, hydroxylapatite column chromatography, Sephadex G-150 gel filtration, Sephadex G-100 gel filtration, and DEAE-Sephadex A-50 gel filtration | Methylomonas aminofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
cell free extract | Methylomonas aminofaciens |
52 | - |
after 16fold purification | Methylomonas aminofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens 77a | D-arabino-hex-3-ulose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
Synonyms | Comment | Organism |
---|---|---|
3-hexulosephosphate synthase | - |
Methylomonas aminofaciens |
D-arabino-3-hexulose 6-phosphate formaldehyde lyase | - |
Methylomonas aminofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Methylomonas aminofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | almost full activity is retained after incubation at 40°C for 30 min, whereas at 80°C for 10 min the enzyme is completely inactivated | Methylomonas aminofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methylomonas aminofaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | stable in neutral to slightly alkaline solutions | Methylomonas aminofaciens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methylomonas aminofaciens | isoelectric focusing | - |
5.1 |