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Literature summary for 4.1.2.43 extracted from

  • Goenrich, M.; Thauer, R.K.; Yurimoto, H.; Kato, N.
    Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis (2005), Arch. Microbiol., 184, 41-48.
    View publication on PubMed
Search for more literature for 4.1.2.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-
Methanosarcina barkeri Fusaro / DSM 804
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4
-
 hexulose-6-phosphate synthase activity of the gene product Fae-Hps, at 30°C, pH 7.0 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
 Methanosarcina barkeri D-arabino-hex-3-ulose 6-phosphate
-
 ?
D-ribulose 5-phosphate + formaldehyde
-
 Methanosarcina barkeri Fusaro / DSM 804 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Fae-Hps protein with hexulose-6-phosphate synthase and formaldehyde activating enzyme activities Methanosarcina barkeri
hexulose-6-phosphate synthase
-
 Methanosarcina barkeri