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Literature summary for 4.1.2.42 extracted from
- Enzymic synthesis of beta-hydroxy-alpha-amino acids based on recombinant D- and L-threonine aldolases (1997), J. Am. Chem. Soc., 119, 11734-11742.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Xanthomonas oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | inactivates | Xanthomonas oryzae |  |
| dimethylformamide | inactivates | Xanthomonas oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.94 | - |
D-threonine | - |
Xanthomonas oryzae | |
| 0.99 | - |
D-allo-threonine | - |
Xanthomonas oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Xanthomonas oryzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas oryzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Xanthomonas oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allo-threonine | - |
Xanthomonas oryzae | glycine + acetaldehyde | - |
r | |
| D-threonine | - |
Xanthomonas oryzae | glycine + acetaldehyde | - |
r | |
| glycine + (4R)-2,2-dimethyl-1,3-dioxolane-4-carbaldehyde | - |
Xanthomonas oryzae | (2R,3R)-2-amino-3-[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid + (2R,3S)-2-amino-3-[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid | - |
r | |
| glycine + (4S)-2,2-dimethyl-1,3-dioxolane-4-carbaldehyde | - |
Xanthomonas oryzae | (2R,3R)-2-amino-3-[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid + (2R,3S)-2-amino-3-[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]-3-hydroxypropanoic acid | - |
? | |
| glycine + 2-methylpropanal | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-4-methylpentanoic acid + (2R,3R)-2-amino-3-hydroxy-4-methylpentanoic acid | - |
r | |
| glycine + 2-nitrobenzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-3-(2-nitrophenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(2-nitrophenyl)propanoic acid | - |
r | |
| glycine + 3-hydroxybenzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-3-(3-hydroxyphenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(3-nitrophenyl)propanoic acid | - |
r | |
| glycine + 4-fluoro-3-nitrobenzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-(4-fluoro-3-nitrophenyl)-3-hydroxypropanoic acid + (2R,3R)-2-amino-3-(4-fluoro-3-nitrophenyl)-3-hydroxypropanoic acid | - |
r | |
| glycine + 4-methylbenzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-3-(4-methylphenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid | - |
r | |
| glycine + 4-nitrobenzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid + (2R,3R)-2-amino-3-hydroxy-3-(4-nitrophenyl)propanoic acid | - |
r | |
| glycine + acetaldehyde | - |
Xanthomonas oryzae | D-threonine + D-allo-threonine | - |
r | |
| glycine + benzaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-3-phenylpropanoic acid + (2R,3R)-2-amino-3-hydroxy-3-phenylpropanoic acid | - |
r | |
| glycine + butyraldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxyhexanoic acid + (2R,3R)-2-amino-3-hydroxyhexanoic acid | - |
r | |
| glycine + hexanaldehyde | - |
Xanthomonas oryzae | (2R,3R)-2-amino-3-hydroxyoctanoic acid + (2R,3S)-2-amino-3-hydroxyoctanoic acid | - |
r | |
| glycine + octanaldehyde | - |
Xanthomonas oryzae | (2R,3R)-2-amino-3-hydroxydecanoic acid + (2R,3S)-2-amino-3-hydroxydecanoic acid | - |
r | |
| glycine + phenylacetaldehyde | - |
Xanthomonas oryzae | (2R,3S)-2-amino-3-hydroxy-4-phenylbutanoic acid + (2R,3R)-2-amino-3-hydroxy-4-phenylbutanoic acid | - |
r | |
| additional information | no activity with but-2-enal, 4-hydroxybenzaldehyde | Xanthomonas oryzae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DTA | - |
Xanthomonas oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Xanthomonas oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
aldol addition | Xanthomonas oryzae |
| 8 | - |
cleavage reaction | Xanthomonas oryzae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required to activate glycine | Xanthomonas oryzae | |
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