Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeras of isoforms LacD.1 and LacD.2. The C-terminus of LacD.1 contributes to its differential enzymatic activity, as replacing of its C-terminal half with 162 amino acid residues from the C-terminus of LacD.2 leads to an enzyme very similarly to LacD.2 in the cleavage of tagatose bisphosphate but over 3 times more efficient than LacD.2 at cleaving fructose bisphosphate. In addition, loop 1 and turn 2 influence the differential enzymatic activity of LacD.1 | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
D-tagatose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
0.199 | - |
D-tagatose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
0.293 | - |
D-fructose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
0.498 | - |
D-fructose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
0.92 | - |
D-tagatose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
1.993 | - |
D-fructose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
2 * 37500, calculated, isoform LacD.1 | Streptococcus pyogenes |
38000 | - |
2 * 38000, calculated, isoform LacD.2 | Streptococcus pyogenes |
67000 | - |
gel filtration, isoform LacD.1 | Streptococcus pyogenes |
71000 | - |
gel filtration, isoform LacD.2 | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Streptococcus pyogenes HSC5 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Fructose 1,6-diphosphate | - |
Streptococcus pyogenes | Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-Fructose 1,6-diphosphate | - |
Streptococcus pyogenes HSC5 | Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-tagatose 1,6-diphosphate | - |
Streptococcus pyogenes | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-tagatose 1,6-diphosphate | - |
Streptococcus pyogenes HSC5 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37500, calculated, isoform LacD.1 | Streptococcus pyogenes |
dimer | 2 * 38000, calculated, isoform LacD.2 | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
LacD.1 | - |
Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.41 | - |
D-fructose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
6.35 | - |
D-fructose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
12.6 | - |
D-fructose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
14.6 | - |
D-tagatose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
18.3 | - |
D-tagatose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
24.3 | - |
D-tagatose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
isoforms LacD.1 and LacD2 | Streptococcus pyogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
D-fructose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
12.8 | - |
D-fructose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
16 | - |
D-tagatose 1,6-diphosphate | isoform LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
42.9 | - |
D-fructose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
100 | - |
D-tagatose 1,6-diphosphate | mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes | |
122 | - |
D-tagatose 1,6-diphosphate | isoform LacD.1, pH 7.5, temperature not specified in the publication | Streptococcus pyogenes |