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Literature summary for 4.1.2.40 extracted from

  • Cusumano, Z.; Caparon, M.
    Adaptive evolution of the Streptococcus pyogenes regulatory aldolase LacD.1 (2013), J. Bacteriol., 195, 1294-1304.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeras of isoforms LacD.1 and LacD.2. The C-terminus of LacD.1 contributes to its differential enzymatic activity, as replacing of its C-terminal half with 162 amino acid residues from the C-terminus of LacD.2 leads to an enzyme very similarly to LacD.2 in the cleavage of tagatose bisphosphate but over 3 times more efficient than LacD.2 at cleaving fructose bisphosphate. In addition, loop 1 and turn 2 influence the differential enzymatic activity of LacD.1 Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.183
-
D-tagatose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
0.199
-
D-tagatose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
0.293
-
D-fructose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
0.498
-
D-fructose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
0.92
-
D-tagatose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
1.993
-
D-fructose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
2 * 37500, calculated, isoform LacD.1 Streptococcus pyogenes
38000
-
2 * 38000, calculated, isoform LacD.2 Streptococcus pyogenes
67000
-
gel filtration, isoform LacD.1 Streptococcus pyogenes
71000
-
gel filtration, isoform LacD.2 Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes HSC5
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose 1,6-diphosphate
-
Streptococcus pyogenes Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-Fructose 1,6-diphosphate
-
Streptococcus pyogenes HSC5 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-tagatose 1,6-diphosphate
-
Streptococcus pyogenes glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-tagatose 1,6-diphosphate
-
Streptococcus pyogenes HSC5 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37500, calculated, isoform LacD.1 Streptococcus pyogenes
dimer 2 * 38000, calculated, isoform LacD.2 Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
LacD.1
-
Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.41
-
D-fructose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
6.35
-
D-fructose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
12.6
-
D-fructose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
14.6
-
D-tagatose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
18.3
-
D-tagatose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
24.3
-
D-tagatose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
isoforms LacD.1 and LacD2 Streptococcus pyogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
D-fructose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
12.8
-
D-fructose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
16
-
D-tagatose 1,6-diphosphate isoform LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
42.9
-
D-fructose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
100
-
D-tagatose 1,6-diphosphate mutant isoform LacD.1, C-terminal half replaced with 162 amino acid residues from the C-terminus of LacD.2, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
122
-
D-tagatose 1,6-diphosphate isoform LacD.1, pH 7.5, temperature not specified in the publication Streptococcus pyogenes