Literature summary for 4.1.2.40 extracted from

Lee, S.J.; Kim, H.S.; Kim, d.o..J.; Yoon, H.J.; Kim, K.H.; Yoon, J.Y.; Suh, S.W.
Crystal structures of LacD from Staphylococcus aureus and LacD.1 from Streptococcus pyogenes: insights into substrate specificity and virulence gene regulation (2011), FEBS Lett., 585, 307-312.

Search for more literature for 4.1.2.40

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2(DE3) cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method	Staphylococcus aureus
sitting drop vapor diffusion method, using	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
L165E	the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate	Staphylococcus aureus
L165E/L275S	the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate	Staphylococcus aureus
L275S	the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q5HE13	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography, HiTrap-Q column chromatography, and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-tagatose 1,6-bisphosphate	-	Staphylococcus aureus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	x-ray crystallography	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
LacD	a class I tagatose-1,6-bisphosphate aldolase	Staphylococcus aureus
tagatose-1,6-bisphosphate aldolase	-	Staphylococcus aureus
TBP aldolase	-	Staphylococcus aureus

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Release 2023.1 (January 2023)