Literature summary for 4.1.2.40 extracted from

Lowkam, C.; Liotard, B.; Sygusch, J.
Structure of a class I tagatose-1,6-biphosphate aldolase: Study into an apparent loss of stereospecificity (2010), J. Biol. Chem., 5, 21143-21152.

Cloned(Commentary)

Cloned (Comment)	Organism
plasmid pKK-223-3 with T7 IPTG-inducible promoter and coding for the lacD2 gene product tagatose-1-6-biphosphate aldolase 2 transformed and overexpressed using the Escherichia coli JM109 strain	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme and native enzyme in complex with dihydroxyacetone-P, by the hanging drop method. Native free TBP aldolase crystallized in the P212121 space group with four protomers in the asymmetric unit and diffracted to 1.87 A resolution. Folding of the polypeptide chain of TBP aldolase corresponds to a (alpha/beta)8 or TIM barrel fold. The liganded crystal structure is isomorphous with the crystal structure of the native aldolase and diffracted to 1.92 A resolution	Streptococcus pyogenes

Crystallization (Comment)

Organism

native enzyme and native enzyme in complex with dihydroxyacetone-P, by the hanging drop method. Native free TBP aldolase crystallized in the P212121 space group with four protomers in the asymmetric unit and diffracted to 1.87 A resolution. Folding of the polypeptide chain of TBP aldolase corresponds to a (alpha/beta)8 or TIM barrel fold. The liganded crystal structure is isomorphous with the crystal structure of the native aldolase and diffracted to 1.92 A resolution

Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by anion-exchange chromatography and gel filtration, to homogeneity	Streptococcus pyogenes

Subunits

Subunits	Comment	Organism
dimer	gel filtration	Streptococcus pyogenes

Synonyms

Synonyms	Comment	Organism
tagatose-1,6-biphosphate aldolase 2	-	Streptococcus pyogenes
TBP aldolase	-	Streptococcus pyogenes

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Release 2023.1 (January 2023)