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Literature summary for 4.1.2.40 extracted from

  • Anderson, R.L.; Markwell, J.P.
    D-Tagatose-1,6-bisphosphate aldolase (class II) from Klebsiella pneumoniae (1982), Methods Enzymol., 90, 232-234.
    View publication on PubMed
Search for more literature for 4.1.2.40

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Klebsiella pneumoniae
NaBH4 no inhibition Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 D-tagatose 1,6-diphosphate
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
CdSO4 activates Klebsiella pneumoniae
CoCl2 activates Klebsiella pneumoniae
KCl activates Klebsiella pneumoniae
MnCl2 activates Klebsiella pneumoniae
NaCl activates Klebsiella pneumoniae
NH4Cl activates Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
157000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Tagatose-1,6-bisphosphate Klebsiella pneumoniae enzyme functions in catabolism of galactitol, inducible enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.07
-
-
 Klebsiella pneumoniae

Storage Stability

Storage Stability Organism
-20°C, 20 mM sodium phosphate buffer, pH 7.5, 10% v/v glycerol, stable for 3 months Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Psicose 1,6-diphosphate no activity Klebsiella pneumoniae Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-Sorbose 1,6-diphosphate no activity Klebsiella pneumoniae Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-Tagatose-1,6-bisphosphate r Klebsiella pneumoniae Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-Tagatose-1,6-bisphosphate in the reverse reaction only the diphosphates of tagatose and fructose are detected Klebsiella pneumoniae Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-Tagatose-1,6-bisphosphate enzyme functions in catabolism of galactitol, inducible enzyme Klebsiella pneumoniae ?
-
 ?
additional information no activity with D-fructose 1-phosphate or L-sorbose 1-phosphate Klebsiella pneumoniae ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.2
-
 Klebsiella pneumoniae