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Literature summary for 4.1.2.4 extracted from

  • Pei, X.; Wang, Q.; Li, C.; Qiu, X.; Xie, K.; Huang, L.; Wang, A.; Zeng, Z.; Xie, T.
    Efficient production of a thermophilic 2-deoxyribose-5-phosphate aldolase in glucose-limited fed-batch cultivations of Escherichia coli by continuous lactose induction strategy (2011), Appl. Biochem. Biotechnol., 165, 416-425.
    View publication on PubMed
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Application

Application Comment Organism
synthesis production of enzyme using a continuous lactose induction strategy. The lactose concentration in the feed medium affects directly the expression of the target protein. The use of 50 g/L in the feed medium results in an expression level of above 30%, and the maximum final enzyme concentration of 16200 U/l. The acetate concentration remains at a low level in the fed-batch phase, less than 0.5 g/l Hyperthermus butylicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Hyperthermus butylicus
expression in Escherichia coli Hyperthermus butylicus

Organism

Organism UniProt Comment Textmining
Hyperthermus butylicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Hyperthermus butylicus D-glyceraldehyde 3-phosphate + acetaldehyde
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 ?

Synonyms

Synonyms Comment Organism
2-Deoxyribose-5-phosphate aldolase
-
 Hyperthermus butylicus
DERA
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 Hyperthermus butylicus

Expression

Organism Comment Expression
Hyperthermus butylicus lactose can induce DERA formation efficiently as isopropyl-beta-D-thiogalactoside. The enzyme concentration increases with the lactose content and reaches a plateau at a concentration of 2.0 g/l up