Literature summary for 4.1.2.38 extracted from

Wiemann, L.O.; Buthe, A.; Klein, M.; van den Wittenboer, A.; Daehne, L.; Ansorge-Schumacher, M.B.
Encapsulation of synthetically valuable biocatalysts into polyelectrolyte multilayer systems (2009), Langmuir, 25, 618-623.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain SG13009 containing plasmid pBALhis6	Pseudomonas fluorescens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	BAL encapsulated in the standard polyelectrolyte multilayer-system retains only about 2% of its native specific activity. An activity decrease of 50% is observed as a result of the deposition of every additional polyelectrolyte layer on the enzyme or when free BAL is dissolved in an aqueous system. Adsorption of two poly(allylamine) hydrochloride/poly(styrene sulfonate) sodium salt double layers on whole cells containing BAL reduces their catalytic activity to 0.01% of the initial value	Pseudomonas fluorescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	4 * 58000, SDS-PAGE	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	biovar I	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.22	-	enzyme-polyelectrolyte multilayer capsules	Pseudomonas fluorescens
10.9	-	native enzyme	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzaldehyde + benzaldehyde	-	Pseudomonas fluorescens	benzoin	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 58000, SDS-PAGE	Pseudomonas fluorescens

Synonyms

Synonyms	Comment	Organism
BAL	-	Pseudomonas fluorescens
benzaldehyde lyase	-	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Pseudomonas fluorescens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas fluorescens	-	-	4.6

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Release 2023.1 (January 2023)