Crystallization (Comment) | Organism |
---|---|
cocrystallization of mutant A28S with inhibitors methyl benzoylphosphonate and benzoylphosphonate. The introduced serine residue of the mutant is phosphorylated by benzoylphosphonate | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
A28S | point mutation converts BAL into a true benzoylformate decarboxylase. Km value for (R)-benzoin is virtually identical to that of the wild-type enzyme but whose kcat value is reduced ca. 10fold. Can decarboxylate benzoylformate, has a substrate spectrum comparable to that of benzoylformate decarboxylase, albeit with reduced activity. Methyl benzoylphosphonate and benzoylphosphonate act as competitive inhibitors in a time- and concentration-dependent manner | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
biovar I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-benzoin | - |
Pseudomonas fluorescens | benzaldehyde + benzaldehyde | - |
? | |
Benzoylformate | - |
Pseudomonas fluorescens | ? | - |
? | |
methyl benzoylphosphonate | - |
Pseudomonas fluorescens | ? | - |
? | |
additional information | shows no reaction with benzoylphosphonate | Pseudomonas fluorescens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAL | - |
Pseudomonas fluorescens |
benzaldehyde lyase | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas fluorescens |