Literature summary for 4.1.2.38 extracted from

Calik, P.; Levent, H.
Effects of pulse feeding of beet molasses on recombinant benzaldehyde lyase production by Escherichia coli BL21(DE3) (2009), Appl. Microbiol. Biotechnol., 85, 65-73.

Search for more literature for 4.1.2.38

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	at fed-batch operation FBO1 (when air-flow rate decreases considerably), feed is given to the system in pulses in such a way that pretreated-beet molasses concentration increases by 10 kg/m3 (containing 2.5 g/L glucose and 2.5 g/L fructose), FBO2 (when fructose and glucose are completely utilized, pretreated-beet molasses is pulse-fed and its concentration increases by 10 g/L), and FBO3 conditions, almost the same BAL volumetric activities are obtained. Fed-batch operation FBO3 is the best feeding strategy because in FBO3 with the decreased amount of pretreated-beet molasses (6 g/L), shift response time from glucose to fructose consumption is avoided, and glucose and fructose consumptions are well correlated with air-flow rate, and the highest cell concentration (8.04 g/L) and BAL (2,315 U/mL) production are obtained (t=24 h) with the highest substrate yield on cell and product formation	synthetic construct

Cloned(Commentary)

Cloned (Comment)	Organism
expressed from pRSETA::balHIS in Escherichia coli BL21(DE3)pLySs	synthetic construct

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	synthetic construct

Organism

Organism	UniProt	Comment	Textmining
synthetic construct	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzoin	-	synthetic construct	benzaldehyde + benzaldehyde	-	?

Synonyms

Synonyms	Comment	Organism
BAL	-	synthetic construct
benzaldehyde lyase	-	synthetic construct

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	synthetic construct

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)