Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Pseudomonas fluorescens |
Crystallization (Comment) | Organism |
---|---|
in 0.1 M Tris-HCl pH 7.5, 0.2 M potassium isothiocyanate, 10% (w/v) polyethylene glycol 1000, and 10% polyethylene glycol 8000 | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | four molecules are located within the protein | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
biovar I | - |
Purification (Comment) | Organism |
---|---|
nickel-affinity HiTrap agarose column chromatography and Sephadex S-200 gel filtration | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + benzaldehyde | - |
Pseudomonas fluorescens | benzoin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAL | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent, four molecules are located within the protein | Pseudomonas fluorescens |