General Stability | Organism |
---|---|
enzyme is unstable when purified more than about 5-fold | Aminobacter aminovorans |
exposure of the enzyme for 30 min at 0°C to buffers of pH values in the region 4 to 9 does not result in loss of activity followed by adjustment of the pH 6.0 | Aminobacter aminovorans |
trimethylamine-grown Pseudomonas aminovorans enzyme is more labile at 40°C than enzyme from methylamine-grown bacteria in French press extracts | Aminobacter aminovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | active at 0.001 mM | Aminobacter aminovorans | |
Mercurials | active at 0.001 mM | Aminobacter aminovorans | |
Triethylamine N-oxide | - |
Aminobacter aminovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Trimethylamine N-oxide | - |
Aminobacter aminovorans | |
140 | - |
Benzyldimethylamine N-oxide | - |
Aminobacter aminovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aminobacter aminovorans | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter aminovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Aminobacter aminovorans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trimethylamine N-oxide = dimethylamine + formaldehyde | non-oxidative, non-hydrolytic cleavage | Aminobacter aminovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyldimethylamine N-oxide | 50% of the activity with trimethylamine N-oxide | Aminobacter aminovorans | ? | - |
? | |
additional information | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | Aminobacter aminovorans | ? | - |
? | |
Trimethylamine N-oxide | - |
Aminobacter aminovorans | Dimethylamine + formaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aminobacter aminovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aminobacter aminovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5.0: about 75% of maximal activity, pH 7.5: about 75% of maximal activity | Aminobacter aminovorans |