Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
K213Q | loss of activity, disappearance of absorption maximum at 420 nm | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(ethylamino)phenol | competitive against DL-threo-3-phenylserine | Pseudomonas putida | |
D-cycloserine | 1 mM, 98% inhibition | Pseudomonas putida | |
DL-3-hydroxy-n-butyrate | 20 mM, 30% inhibition | Pseudomonas putida | |
DL-3-hydroxynorvaline | 20 mM, 49% inhibition | Pseudomonas putida | |
DL-3-hydroxyphenylethylamine | 20 mM, 79% inhibition | Pseudomonas putida | |
hydroxylamine | 1 mM, 97% inhibition | Pseudomonas putida | |
L-3-phenyllactate | 10 mM, 39% inhibition | Pseudomonas putida | |
phenylhydrazine | 1 mM, 52% inhibition | Pseudomonas putida | |
Semicarbazide | 1 mM, 97% inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-threo-3-phenylserine | pH 8.5, 30°C | Pseudomonas putida | |
4.6 | - |
L-erythro-3-phenylserine | pH 8.5, 30°C | Pseudomonas putida | |
22 | - |
L-allo-Thr | pH 8.5, 30°C | Pseudomonas putida | |
29 | - |
L-Thr | pH 8.5, 30°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37400 | - |
6 * 37400, calculation from nucleotide sequence | Pseudomonas putida |
38000 | - |
6 * 38000, SDS-PAGE | Pseudomonas putida |
210000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | inducible enzyme | ? | - |
? | |
additional information | Pseudomonas putida 24. Jan | inducible enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q59IT3 | strain 24-1 | - |
Pseudomonas putida 24. Jan | Q59IT3 | strain 24-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.3 | - |
reaction with glycine and benzaldehyde | Pseudomonas putida |
300 | - |
cleavage of L-threo-3-phenylserine | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, pH 7.2, 10 mM TES buffer, 0.01% 2-mercaptoethanol, 0.05 mM pyridoxal 5'-phosphate, 30% glycerol, stable for several months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + benzaldehyde | - |
Pseudomonas putida | L-threo-3-phenylserine + L-erythro-3-phenylserine | - |
r | |
glycine + benzaldehyde | - |
Pseudomonas putida 24. Jan | L-threo-3-phenylserine + L-erythro-3-phenylserine | - |
r | |
L-allo-Thr | - |
Pseudomonas putida | glycine + acetaldehyde | - |
? | |
L-erythro-3-phenylserine | no activity with D-erythro-3-phenylserine | Pseudomonas putida | glycine + benzaldehyde | - |
r | |
L-Thr | - |
Pseudomonas putida | glycine + acetaldehyde | - |
? | |
L-Thr | - |
Pseudomonas putida 24. Jan | glycine + acetaldehyde | - |
? | |
L-threo-3-phenylserine | no activity with D-threo-3-phenylserine | Pseudomonas putida | glycine + benzaldehyde | - |
r | |
L-threo-3-phenylserine | no activity with D-threo-3-phenylserine | Pseudomonas putida 24. Jan | glycine + benzaldehyde | - |
r | |
additional information | inducible enzyme | Pseudomonas putida | ? | - |
? | |
additional information | no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine | Pseudomonas putida | ? | - |
? | |
additional information | inducible enzyme | Pseudomonas putida 24. Jan | ? | - |
? | |
additional information | no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine | Pseudomonas putida 24. Jan | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 38000, SDS-PAGE | Pseudomonas putida |
hexamer | 6 * 37400, calculation from nucleotide sequence | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
580 | - |
L-Thr | pH 8.5, 30°C | Pseudomonas putida | |
1300 | - |
L-allo-Thr | pH 8.5, 30°C | Pseudomonas putida | |
2300 | - |
L-threo-3-phenylserine | pH 8.5, 30°C | Pseudomonas putida | |
7900 | - |
L-erythro-3-phenylserine | pH 8.5, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reaction with glycine and benzyldehyde | Pseudomonas putida |
8.5 | - |
reaction with L-threo-3-phenylserine | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | 30°C, 10 min, stable | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 0.7 mol of pyridoxal 5'-phosphate per mol of subunit, K213 of the enzyme probably forms a Schiff base with pyridoxal 5'-phosphate | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
3-(ethylamino)phenol | - |
Pseudomonas putida |