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Literature summary for 4.1.2.25 extracted from

  • Wang, Y.; Li, Y.; Yan, H.
    Mechanism of dihydroneopterin aldolase: functional roles of the conserved active site glutamate and lysine residues (2006), Biochemistry, 45, 15232-15239.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E21A strongly reduced kcat Escherichia coli
E22A strongly reduced kcat Staphylococcus aureus
E73A strongly reduced kcat Escherichia coli
E74A mutation causes dramatic changes in the affinities of the enzyme for the substrate or product analogues or the rate constants Staphylococcus aureus
K100A strongly reduced kcat Staphylococcus aureus
K100Q strongly reduced kcat Staphylococcus aureus
K98A strongly reduced kcat Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00076
-
7,8-dihydro-L-monapterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0016
-
7,8-dihydroneopterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0024
-
7,8-dihydroneopterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0029
-
7,8-dihydro-L-monapterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0039
-
7,8-dihydroneopterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.004
-
7,8-dihydro-L-monapterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0046
-
7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0055
-
7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0058
-
7,8-dihydroneopterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0074
-
7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0095
-
7,8-dihydro-L-monapterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
8
-
7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
9.7
-
7,8-dihydroneopterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
9.8
-
7,8-dihydro-L-monapterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Bio-Gel A-0.5m gel filtration Escherichia coli
Ni-NTA column chromatography and Bio-Gel A-0.5m gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-hydroxymethyl-7,8-dihydropterin
-
Staphylococcus aureus ?
-
?
6-hydroxymethyl-7,8-dihydropterin
-
Escherichia coli ?
-
?
7,8-dihydro-L-monapterin
-
Staphylococcus aureus ?
-
?
7,8-dihydro-L-monapterin
-
Escherichia coli ?
-
?
7,8-dihydroneopterin
-
Staphylococcus aureus 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
?
7,8-dihydroneopterin
-
Escherichia coli 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
?

Synonyms

Synonyms Comment Organism
DHNA
-
Staphylococcus aureus
DHNA
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000022
-
7,8-dihydroneopterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0000023
-
7,8-dihydro-L-monapterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0000043
-
7,8-dihydroneopterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0000051
-
7,8-dihydro-L-monapterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0000053
-
7,8-dihydro-L-monapterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0000057
-
7,8-dihydro-L-monapterin mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.000006
-
7,8-dihydro-L-monapterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0000065
-
7,8-dihydroneopterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.0000065
-
7,8-dihydro-L-monapterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.0000093
-
7,8-dihydroneopterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.000016
-
7,8-dihydroneopterin mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.00073
-
7,8-dihydroneopterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.01
-
7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.045
-
7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
0.082
-
7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
0.089
-
7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
-
Staphylococcus aureus
9.6
-
-
Escherichia coli