Protein Variants | Comment | Organism |
---|---|---|
E21A | strongly reduced kcat | Escherichia coli |
E22A | strongly reduced kcat | Staphylococcus aureus |
E73A | strongly reduced kcat | Escherichia coli |
E74A | mutation causes dramatic changes in the affinities of the enzyme for the substrate or product analogues or the rate constants | Staphylococcus aureus |
K100A | strongly reduced kcat | Staphylococcus aureus |
K100Q | strongly reduced kcat | Staphylococcus aureus |
K98A | strongly reduced kcat | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00076 | - |
7,8-dihydro-L-monapterin | mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0016 | - |
7,8-dihydroneopterin | mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0024 | - |
7,8-dihydroneopterin | mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0029 | - |
7,8-dihydro-L-monapterin | mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0039 | - |
7,8-dihydroneopterin | mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.004 | - |
7,8-dihydro-L-monapterin | mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0046 | - |
7,8-dihydroneopterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0055 | - |
7,8-dihydro-L-monapterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0058 | - |
7,8-dihydroneopterin | mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0074 | - |
7,8-dihydroneopterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0095 | - |
7,8-dihydro-L-monapterin | mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
8 | - |
7,8-dihydro-L-monapterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
9.7 | - |
7,8-dihydroneopterin | mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
9.8 | - |
7,8-dihydro-L-monapterin | mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography and Bio-Gel A-0.5m gel filtration | Escherichia coli |
Ni-NTA column chromatography and Bio-Gel A-0.5m gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxymethyl-7,8-dihydropterin | - |
Staphylococcus aureus | ? | - |
? | |
6-hydroxymethyl-7,8-dihydropterin | - |
Escherichia coli | ? | - |
? | |
7,8-dihydro-L-monapterin | - |
Staphylococcus aureus | ? | - |
? | |
7,8-dihydro-L-monapterin | - |
Escherichia coli | ? | - |
? | |
7,8-dihydroneopterin | - |
Staphylococcus aureus | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
7,8-dihydroneopterin | - |
Escherichia coli | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHNA | - |
Staphylococcus aureus |
DHNA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000022 | - |
7,8-dihydroneopterin | mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0000023 | - |
7,8-dihydro-L-monapterin | mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0000043 | - |
7,8-dihydroneopterin | mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0000051 | - |
7,8-dihydro-L-monapterin | mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0000053 | - |
7,8-dihydro-L-monapterin | mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0000057 | - |
7,8-dihydro-L-monapterin | mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.000006 | - |
7,8-dihydro-L-monapterin | mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0000065 | - |
7,8-dihydroneopterin | mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.0000065 | - |
7,8-dihydro-L-monapterin | mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.0000093 | - |
7,8-dihydroneopterin | mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.000016 | - |
7,8-dihydroneopterin | mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.00073 | - |
7,8-dihydroneopterin | mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.01 | - |
7,8-dihydro-L-monapterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.045 | - |
7,8-dihydroneopterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Staphylococcus aureus | |
0.082 | - |
7,8-dihydroneopterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli | |
0.089 | - |
7,8-dihydro-L-monapterin | wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
- |
Staphylococcus aureus |
9.6 | - |
- |
Escherichia coli |