Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain NR7 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F33I/D58N/Q72H/A75V/V85A/V154F | higher activity compared to the wild type enzyme | Escherichia coli |
additional information | mutant NR8.276-2 exhibits a 60fold improvement in the ratio kcat/KM relative to that of the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-erythrose 4-phosphate | mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C | Escherichia coli | |
0.57 | - |
D-erythrose 4-phosphate | wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose 4B resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-D-galactonate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
pyruvate + D-erythrose 4-phosphate | - |
Escherichia coli | 3-deoxy-D-arabino-heptulosonic acid 7-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxy-6-phosphogalactonate aldolase | - |
Escherichia coli |
DgoA | - |
Escherichia coli |
KDPGal aldolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
D-erythrose 4-phosphate | wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C | Escherichia coli | |
2.5 | - |
D-erythrose 4-phosphate | mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C | Escherichia coli |