Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
8 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Picrophilus torridus |
120000 | - |
gel filtration | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration | Picrophilus torridus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:glucose-grown cell | - |
Picrophilus torridus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
crude extract, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
48 | - |
after 343fold purification, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Picrophilus torridus | pyruvate + glyceraldehyde-3-phosphate | - |
? | |
2-dehydro-3-deoxy-D-gluconate | the enzyme is highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate | Picrophilus torridus | pyruvate + glyceraldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 32000, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxygluconate-specific aldolase | - |
Picrophilus torridus |
KDG-specific aldolase | - |
Picrophilus torridus |
KDGA | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | at 70°C, the enzyme does not lose activity upon incubation for 2 h. The half-lives of the enzyme at 80°C and 90°C are 20 min and 15 min, respectively | Picrophilus torridus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
26.7 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Picrophilus torridus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | - |
Picrophilus torridus |
General Information | Comment | Organism |
---|---|---|
metabolism | Picrophilus torridus degrades glucose via a strictly nonphosphorylative Entner-Doudoroff pathway with a 2-keto-3-deoxygluconate-specific aldolase | Picrophilus torridus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
89 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |