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Literature summary for 4.1.2.20 extracted from

  • Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.
    The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase (2010), J. Bacteriol., 192, 964-974.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells Picrophilus torridus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
2-dehydro-3-deoxy-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus
8
-
2-dehydro-3-deoxy-6-phospho-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, SDS-PAGE Picrophilus torridus
120000
-
gel filtration Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration Picrophilus torridus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:glucose-grown cell
-
Picrophilus torridus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
crude extract, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus
48
-
after 343fold purification, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate
-
Picrophilus torridus pyruvate + glyceraldehyde-3-phosphate
-
?
2-dehydro-3-deoxy-D-gluconate the enzyme is highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate Picrophilus torridus pyruvate + glyceraldehyde
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 32000, SDS-PAGE Picrophilus torridus

Synonyms

Synonyms Comment Organism
2-keto-3-deoxygluconate-specific aldolase
-
Picrophilus torridus
KDG-specific aldolase
-
Picrophilus torridus
KDGA
-
Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Picrophilus torridus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 at 70°C, the enzyme does not lose activity upon incubation for 2 h. The half-lives of the enzyme at 80°C and 90°C are 20 min and 15 min, respectively Picrophilus torridus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
2-dehydro-3-deoxy-6-phospho-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus
26.7
-
2-dehydro-3-deoxy-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Picrophilus torridus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5
-
Picrophilus torridus

General Information

General Information Comment Organism
metabolism Picrophilus torridus degrades glucose via a strictly nonphosphorylative Entner-Doudoroff pathway with a 2-keto-3-deoxygluconate-specific aldolase Picrophilus torridus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
2-dehydro-3-deoxy-6-phospho-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus
89
-
2-dehydro-3-deoxy-D-gluconate at 60°C in 50 mM sodium phosphate buffer (pH 6.2) Picrophilus torridus