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Literature summary for 4.1.2.18 extracted from

  • Dahms, A.S.; Anderson, R.L.
    D-Fucose metabolism in a pseudomonad. IV. Cleavage of 2-keto-3-deoxy-D-fuconate to pyruvate and D-lactaldehyde by 2-keto-3-deoxy-L-arabonate aldolase (1972), J. Biol. Chem., 247, 2238-2241.
    View publication on PubMed
Search for more literature for 4.1.2.18

General Stability

General Stability Organism
thiols stabilize during purification Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp.
2.9
-
 2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required Pseudomonas sp.
Co2+ 95% of the activation with Mn2+ Pseudomonas sp.
Mg2+ divalent cation required Pseudomonas sp.
Mg2+ 54% of the activity with Mn2+ Pseudomonas sp.
Mn2+ divalent cation required Pseudomonas sp.
Mn2+ most effective Pseudomonas sp.
Ni2+ divalent cation required Pseudomonas sp.
Ni2+ 45% of the activity with Mn2+ Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. enzyme activity increases 3fold by growth on D-fucose or L-arabinose ?
-
 ?
additional information Pseudomonas sp. MSU-1 enzyme activity increases 3fold by growth on D-fucose or L-arabinose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MSU-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.63
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 0.1 M bicine, 0.14 mM 2-thioethanol, 3 weeks, 70% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp. Pyruvate + D-lactaldehyde
-
 ?
2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp. MSU-1 Pyruvate + D-lactaldehyde
-
 ?
2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp. Pyruvate + glycolaldehyde
-
 ?
2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp. MSU-1 Pyruvate + glycolaldehyde
-
 ?
additional information enzyme activity increases 3fold by growth on D-fucose or L-arabinose Pseudomonas sp. ?
-
 ?
additional information enzyme activity increases 3fold by growth on D-fucose or L-arabinose Pseudomonas sp. MSU-1 ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-life: 15 min Pseudomonas sp.