Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
expressed in Escherichia coli BL21(DE3) cells | Pyricularia grisea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the specific activity of the Bacillus cereus aldolase is partially restored after EDTA inactivation by Co2+ and Cd2+, as well as Zn2+, but not significantly restored by Cu2+, Mn2+, Mg2+, or Ni2+ | Bacillus cereus | |
EDTA | the activity of the EDTA-inactivated enzyme increases more than 6fold upon the addition of 0.002 mM Zn2+ and increases by a factor 2 with the addition of 0.002 mM Co2+. The addition of Cu2+, Ni2+, Cd2+, Mn2+, or Mg2+ does not reactivate the enzyme significantly | Mycobacterium tuberculosis | |
EDTA | the specific activity of the recombinant Pseudomonas aeruginosa aldolase is 5.5times higher after inactivation by EDTA followed by the addition of 0.02 mM cobalt chloride, and increases more than 2fold after EDTA inactivation followed by the addition of 0.1 mM manganese(II)-chloride | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0279 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Mycobacterium tuberculosis | |
0.035 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pseudomonas aeruginosa | |
0.051 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pyricularia grisea | |
0.45 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the addition of Co2+ to the purified Bacillus cereus aldolase does not increase the relatively low specific activity of this recombinant enzyme | Bacillus cereus | |
Zn2+ | contains 0.16 zinc atoms per subunit | Pseudomonas aeruginosa | |
Zn2+ | contains 0.49 zinc atoms per subunit | Mycobacterium tuberculosis | |
Zn2+ | contains 0.83 zinc atoms per subunit | Pyricularia grisea | |
Zn2+ | contains 0.97 zinc atoms per subunit | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30540 | - |
1 * 30671 + 1 * 30540, mass spectrometry | Bacillus cereus |
30671 | - |
1 * 30671 + 1 * 30540, mass spectrometry | Bacillus cereus |
36411 | - |
4 * 36411, mass spectrometry | Mycobacterium tuberculosis |
38441 | - |
4 * 38441, mass spectrometry | Pseudomonas aeruginosa |
39644 | - |
1 * 39775 + 1 * 39644, mass spectrometry | Pyricularia grisea |
39775 | - |
1 * 39775 + 1 * 39644, mass spectrometry | Pyricularia grisea |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetone | Pyricularia grisea |
Acetone | more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone | Pseudomonas aeruginosa |
Acetone | more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone | Bacillus cereus |
Acetone | more than 50% of the enzyme activity is retained in the presence of 25% (v/v) acetone | Mycobacterium tuberculosis |
acetonitrile | more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetonitrile | Bacillus cereus |
acetonitrile | more than 50% of the enzyme activity is retained in the presence of 10% (v/v) acetonitrile | Mycobacterium tuberculosis |
acetonitrile | more than 50% of the enzyme activity is retained in the presence of 15% (v/v) acetonitrile | Pseudomonas aeruginosa |
acetonitrile | more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) acetonitrile | Pyricularia grisea |
dimethylformamide | more than 50% of the enzyme activity is retained in the presence of 10% (v/v) dimethylformamide | Pyricularia grisea |
dimethylformamide | more than 50% of the enzyme activity is retained in the presence of 20% (v/v) dimethylformamide | Mycobacterium tuberculosis |
dimethylformamide | more than 50% of the enzyme activity is retained in the presence of 35% (v/v) dimethylformamide | Pseudomonas aeruginosa |
dimethylformamide | more than 50% of the enzyme activity is retained in the presence of 50% (v/v) dimethylformamide | Bacillus cereus |
DMSO | 167% activity in the presence of 50% (v/v) DMSO | Bacillus cereus |
DMSO | more than 50% of the enzyme activity is retained in the presence of 20% (v/v) DMSO | Pyricularia grisea |
DMSO | more than 50% of the enzyme activity is retained in the presence of 50% (v/v) DMSO | Pseudomonas aeruginosa |
DMSO | more than 50% of the enzyme activity is retained in the presence of 50% (v/v) DMSO | Mycobacterium tuberculosis |
tert-Butanol | more than 50% of the enzyme activity is retained in the presence of 15% (v/v) tert-butanol | Pseudomonas aeruginosa |
tert-Butanol | more than 50% of the enzyme activity is retained in the presence of 20% (v/v) tert-butanol | Mycobacterium tuberculosis |
tert-Butanol | more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) tert-butanol | Bacillus cereus |
tert-Butanol | more than 50% of the enzyme activity is retained in the presence of about 5% (v/v) tert-butanol | Pyricularia grisea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus ATCC 10987 | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pyricularia grisea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography | Pseudomonas aeruginosa |
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography | Bacillus cereus |
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography | Mycobacterium tuberculosis |
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sepharose Q column chromatography | Pyricularia grisea |
Storage Stability | Organism |
---|---|
4°C, in 50 mM HEPES buffer with pH 7.3 supplemented with ZnCl2, 24 h, 40% loss of activity | Bacillus cereus |
4°C, in 50 mM HEPES buffer with pH 7.3 supplemented with ZnCl2, several days, the enzyme remains stable | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Pseudomonas aeruginosa | dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | - |
Bacillus cereus | dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | - |
Mycobacterium tuberculosis | dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | - |
Pyricularia grisea | dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | - |
Bacillus cereus ATCC 10987 | dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 30671 + 1 * 30540, mass spectrometry | Bacillus cereus |
heterodimer | 1 * 39775 + 1 * 39644, mass spectrometry | Pyricularia grisea |
homotetramer | 4 * 36411, mass spectrometry | Mycobacterium tuberculosis |
homotetramer | 4 * 38441, mass spectrometry | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
class II fructose 1,6-bisphosphate aldolase | - |
Pseudomonas aeruginosa |
class II fructose 1,6-bisphosphate aldolase | - |
Bacillus cereus |
class II fructose 1,6-bisphosphate aldolase | - |
Mycobacterium tuberculosis |
class II fructose 1,6-bisphosphate aldolase | - |
Pyricularia grisea |
FBP aldolase | - |
Pseudomonas aeruginosa |
FBP aldolase | - |
Bacillus cereus |
FBP aldolase | - |
Mycobacterium tuberculosis |
FBP aldolase | - |
Pyricularia grisea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | the aldolase loses 25% of its activity after 10 min of incubation at 45°C and loses almost all its activity after a10 min incubation at 60°C | Pyricularia grisea |
60 | - |
the aldolase loses almost all its activity after a 10 min incubation at 60°C | Mycobacterium tuberculosis |
60 | - |
the aldolase loses almost all its activity after a10 min incubation at 60°C | Pseudomonas aeruginosa |
90 | - |
the aldolase retains almost half of its activity after 10 min of incubation at 90°C | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.53 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pseudomonas aeruginosa | |
2.95 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Bacillus cereus | |
30.1 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Mycobacterium tuberculosis | |
45.7 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pyricularia grisea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Mycobacterium tuberculosis |
7.8 | - |
- |
Pyricularia grisea |
8 | - |
- |
Bacillus cereus |
8.5 | - |
- |
Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Bacillus cereus | |
0.44 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pseudomonas aeruginosa | |
9 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Pyricularia grisea | |
10.8 | - |
D-fructose 1,6-bisphosphate | in Tris-HCl pH 8.0, at 30°C | Mycobacterium tuberculosis |