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Literature summary for 4.1.2.13 extracted from
- MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity (2008), FEMS Microbiol. Lett., 281, 36-41.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | citrate or phosphoenolpyruvate show no effect on the FBP activity of MJ0400-His6 | Methanocaldococcus jannaschii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning, overexpression of C-terminally His-tagged MJ0400 enzyme in Escherichia coli Rosetta2(DE3)pLysS cells | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-erythrose 4-phosphate | competitive | Methanocaldococcus jannaschii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
D-fructose-1,6-bisphosphate | at 50°C | Methanocaldococcus jannaschii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose-1,6-bisphosphate | Methanocaldococcus jannaschii | - |
glyceraldehyde-3-phosphate + dihydroxyacetone phosphate | - |
r | |
| additional information | Methanocaldococcus jannaschii | no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose-1,6-bisphosphate | - |
Methanocaldococcus jannaschii | glyceraldehyde-3-phosphate + dihydroxyacetone phosphate | - |
r | |
| additional information | no activity of MJ0400-His6 is measured with 10 mM fructose-1-phosphate as a substrate. MJ0400 also acts as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, this indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in Methanocaldococcus jannaschii | Methanocaldococcus jannaschii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBP aldolase | - |
Methanocaldococcus jannaschii |
| fructose-1,6-bisphosphate aldolase | - |
Methanocaldococcus jannaschii |
| MJ0400-His6 | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at, temperature optimum for the specific acitivity of MJ0400-His6 | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
displays heat stability with a half-life of 1 h at 100°C | Methanocaldococcus jannaschii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
D-erythrose 4-phosphate | - |
Methanocaldococcus jannaschii | |
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