Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21pLysS cells | Leishmania mexicana |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method using 15% polyethylene glycol 5000 MME in 20 mM sodium phosphate pH 4.0 | Leishmania mexicana |
Protein Variants | Comment | Organism |
---|---|---|
G312A | mutation does not perturb ligand binding in the active site | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-hexitol-1,6-bisphosphate | - |
Leishmania mexicana | |
D-mannitol-1,6-bisphosphate | competitive inhibitor | Leishmania mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | Q9U5N6 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel chelating affinity chromatography and gel filtration | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Leishmania mexicana | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Leishmania mexicana |