Literature summary for 4.1.2.13 extracted from

Jayanthi Bai, N.; Ramachandra Pai, M.; Suryanarayana Murthy, P.; Venkitasubramanian, T.A.
Fructose-bisphosphate aldolases from Mycobacteria (1982), Methods Enzymol., 90, 241-250.

Search for more literature for 4.1.2.13

General Stability

General Stability	Organism
carboxypeptidase A treatment results in 20% inactivation. Complete protection by D-fructose 1,6-bisphosphate	Mycolicibacterium smegmatis

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	aldolase class II	Mycobacterium tuberculosis
2,2'-dipyridyl	aldolase class II	Mycobacterium tuberculosis
8-hydroxyquinoline	aldolase class II	Mycobacterium tuberculosis
Co2+	-	Mycolicibacterium smegmatis
Cu2+	-	Mycolicibacterium smegmatis
EDTA	-	Mycobacterium tuberculosis
EDTA	insensitive	Mycolicibacterium smegmatis
Fe2+	-	Mycolicibacterium smegmatis
Mn2+	-	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	D-fructose 1,6-bisphosphate	-	Mycolicibacterium smegmatis
0.08	-	D-fructose 1,6-bisphosphate	aldolase class II	Mycobacterium tuberculosis
10	-	fructose 1-phosphate	-	Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	no effect	Mycolicibacterium smegmatis
K+	stimulates	Mycobacterium tuberculosis
Mg2+	no effect	Mycolicibacterium smegmatis
Zn2+	no effect	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	4 * 40000, SDS-PAGE	Mycolicibacterium smegmatis
158000	-	gel filtration	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	H37Rv, NCTC, 7416: aldolase class I	-
Mycobacterium tuberculosis	-	NCTC, 7416: aldolase class II	-
Mycolicibacterium smegmatis	-	CDC No. 46	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mycolicibacterium smegmatis
additional information	-	-	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-20°C, stable for 3 weeks	Mycolicibacterium smegmatis
-20°C, stable for 4 weeks	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Mycolicibacterium smegmatis	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	-	Mycobacterium tuberculosis	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-Fructose 1-phosphate	-	Mycolicibacterium smegmatis	Glycerone phosphate + D-glyceraldehyde	-	?
D-Fructose 1-phosphate	no activity	Mycobacterium tuberculosis	Glycerone phosphate + D-glyceraldehyde	-	?
Glycerone phosphate + D-glyceraldehyde 3-phosphate	aldol synthesizing activity is only 1.5% of fructose 1,6-diphosphate cleavage	Mycolicibacterium smegmatis	D-Fructose 1,6-bisphosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 40000, SDS-PAGE	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	-	Mycolicibacterium smegmatis
8.6	-	aldolase class II	Mycobacterium tuberculosis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	30 min, 90% loss of activity	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)