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Literature summary for 4.1.1.B4 extracted from

  • Gu, X.; Glushka, J.; Lee, S.G.; Bar-Peled, M.
    Biosynthesis of a new UDP-sugar, UDP-2-acetamido-2-deoxyxylose, in the human pathogen Bacillus cereus subspecies cytotoxis NVH 391-98 (2010), J. Biol. Chem., 285, 24825-24833.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.B4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus cytotoxicus NVH 391-98

Inhibitors

Inhibitors Comment Organism Structure
NADH 0.5 mM, presence of 0.5 mM NAD+, no residual activity Bacillus cytotoxicus NVH 391-98
NADPH 0.5 mM, presence of 0.5 mM NAD+, 92% residual activity Bacillus cytotoxicus NVH 391-98
UDP 0.5 mM, presence of 0.5 mM NAD+, 48% residual activity Bacillus cytotoxicus NVH 391-98
UDP-glucuronic acid 0.5 mM, presence of 0.5 mM NAD+, 70% residual activity Bacillus cytotoxicus NVH 391-98
UMP 0.5 mM, presence of 0.5 mM NAD+, 93% residual activity Bacillus cytotoxicus NVH 391-98

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 UDP-N-acetylglucosaminuronic acid pH 8.2, 37°C Bacillus cytotoxicus NVH 391-98

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Bacillus cytotoxicus NVH 391-98
70200
-
 gel filtration Bacillus cytotoxicus NVH 391-98

Organism

Organism UniProt Comment Textmining
Bacillus cytotoxicus NVH 391-98 A7GKX7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Bacillus cytotoxicus NVH 391-98

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetylglucosaminuronic acid = UDP-N-acetylxylosamine + CO2 exogenous NAD+ required. The enzyme-bound NAD+ is not released as NADH during the C-4 oxidation/reduction cycle Bacillus cytotoxicus NVH 391-98
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDP-N-acetyl-glucosamine 6-dehydrogenase and UDP-N-acetylglucosaminuronic acid decarboxylase catalyze the enzymatic conversion of UDP-N-acetyl-glucosamine to UDP-N-acetylxylosamine. No substrate: UDP-glucuronic acid and UDP-galacturonic acid Bacillus cytotoxicus NVH 391-98 ?
-
 ?
UDP-N-acetylglucosaminuronic acid
-
 Bacillus cytotoxicus NVH 391-98 UDP-N-acetylxylosamine + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer x * 35000, SDS-PAGE Bacillus cytotoxicus NVH 391-98

Synonyms

Synonyms Comment Organism
UXNAcS
-
 Bacillus cytotoxicus NVH 391-98

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus cytotoxicus NVH 391-98

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
 UDP-N-acetylglucosaminuronic acid pH 8.2, 37°C Bacillus cytotoxicus NVH 391-98

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Bacillus cytotoxicus NVH 391-98

pH Range

pH Minimum pH Maximum Comment Organism
4
-
 no activity below Bacillus cytotoxicus NVH 391-98

Cofactor

Cofactor Comment Organism Structure
additional information NADP+ cannot substitute for NAD+ Bacillus cytotoxicus NVH 391-98
NAD+ exogenous NAD+ required. The enzyme-bound NAD+ is not released as NADH during the C-4 oxidation/reduction cycle Bacillus cytotoxicus NVH 391-98

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7
-
 UDP-N-acetylglucosaminuronic acid pH 8.2, 37°C Bacillus cytotoxicus NVH 391-98