Literature summary for 4.1.1.98 extracted from

Rangarajan, E.S.; Li, Y.; Iannuzzi, P.; Tocilj, A.; Hung, L.W.; Matte, A.; Cygler, M.
Crystal structure of a dodecameric FMN-dependent UbiX-like decarboxylase (Pad1) from Escherichia coli O157: H7 (2004), Protein Sci., 13, 3006-3016.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.2 M LiSO4, and 0.1 M Hepes buffer (pH 7.0)	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23175	-	12 * 23175, electron spray ionization mass spectrometry	Escherichia coli
23183	-	12 * 23183, calculated from amino acid sequence	Escherichia coli
295000	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 4-hydroxy-3-polyprenylbenzoate	Escherichia coli	-	a 2-polyprenylphenol + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P69772	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superose 12 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-octaprenyl-4-hydroxybenzoate	-	Escherichia coli	2-octaprenylphenol + CO2	-	?
a 4-hydroxy-3-polyprenylbenzoate	-	Escherichia coli	a 2-polyprenylphenol + CO2	-	?

Subunits

Subunits	Comment	Organism
homododecamer	12 * 23175, electron spray ionization mass spectrometry	Escherichia coli
homododecamer	12 * 23183, calculated from amino acid sequence	Escherichia coli

Synonyms

Synonyms	Comment	Organism
3-octaprenyl-4-hydroxybenzoate carboxy-lyase	-	Escherichia coli
PAD1	-	Escherichia coli
ubiX	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FMN	dependent on	Escherichia coli

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Release 2023.1 (January 2023)