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Literature summary for 4.1.1.96 extracted from

  • Lee, J.; Sperandio, V.; Frantz, D.E.; Longgood, J.; Camilli, A.; Phillips, M.A.; Michael, A.J.
    An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae (2009), J. Biol. Chem., 284, 9899-9907.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Vibrio vulnificus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
0.27
-
 carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nitrilotriacetic acid affinity resin column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxynorspermidine carboxynorspermidine is the primary substrate and is decarboxylated 20fold more efficiently than carboxyspermidine Vibrio vulnificus bis(3-aminopropyl)amine + CO2
-
 ?
carboxyspermidine
-
 Vibrio vulnificus spermidine + CO2
-
 ?
L-ornithine the enzyme also exhibits some activity with L-ornithine Vibrio vulnificus ?
-
 ?
additional information the enzyme exhibits no activity with L-arginine, L-lysine, and meso-diaminopimelate Vibrio vulnificus ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 45000, SDS-PAGE Vibrio vulnificus

Synonyms

Synonyms Comment Organism
CANSDC
-
 Vibrio vulnificus
carboxyspermidine decarboxylase
-
 Vibrio vulnificus
nspC
-
 Vibrio vulnificus
VC1623
-
 Vibrio vulnificus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
 carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
20.1
-
 carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Vibrio vulnificus

General Information

General Information Comment Organism
metabolism the CANSDH/CANSDC alternative polyamine biosynthetic pathway is able to synthesize both sym-norspermidine and spermidine Vibrio vulnificus
physiological function the enzyme is essential for biofilm formation Vibrio vulnificus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2
-
 carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
74
-
 carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus