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Literature summary for 4.1.1.88 extracted from

  • Byun, H.S.; Kim, Y.S.
    Subunit organization of bacterial malonate decarboxylases: The smallest delta subunit as an acyl-carrier protein (1997), J. Biochem. Mol. Biol., 30, 132-137.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
diethyldicarbonate 1 mM, 114% of initial activity Pseudomonas fluorescens
N-Acetylimidazole 1 mM, 117% of initial activity Pseudomonas fluorescens
pyridoxal 5'phosphate 1 mM, 112% of initial activity Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid) 0.5 mM, 1.5% residual activity Acinetobacter calcoaceticus
Bromoacetate 0.5 mM, 0.9% residual activity Acinetobacter calcoaceticus
Bromoacetate 0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity Pseudomonas fluorescens
diethyldicarbonate 1 mM, 91% residual activity Acinetobacter calcoaceticus
diethyldicarbonate 1 mM, 88% residual activity Pseudomonas fluorescens
iodoacetamide 0.5 mM, 58% residual activity Acinetobacter calcoaceticus
N-Acetylimidazole 1 mM, 95% residual activity Acinetobacter calcoaceticus
N-Acetylimidazole 1 mM, 95% residual activity Pseudomonas fluorescens
N-ethylmaleimide 0.5 mM, 0.1% residual activity Acinetobacter calcoaceticus
N-ethylmaleimide 0.5 mM, 0.2% residual activity Pseudomonas fluorescens
pyridoxal 5'phosphate 1 mM, 82% residual activity Acinetobacter calcoaceticus
pyridoxal 5'phosphate 1 mM, 92% residual activity Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit Acinetobacter calcoaceticus
11000
-
 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit Pseudomonas fluorescens
25000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit Acinetobacter calcoaceticus
30000
-
 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit Pseudomonas fluorescens
32000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit Acinetobacter calcoaceticus
33000
-
 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit Pseudomonas fluorescens
65000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit Acinetobacter calcoaceticus
65000
-
 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit Pseudomonas fluorescens
185000
-
 gel filtration Pseudomonas fluorescens
185000
-
 gel filtration Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 grown on malonate
-
Pseudomonas fluorescens
-
 grown on malonate
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group Pseudomonas fluorescens
additional information the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group Acinetobacter calcoaceticus

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens
-
 Acinetobacter calcoaceticus

Renatured (Commentary)

Renatured (Comment) Organism
reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively Pseudomonas fluorescens
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity Pseudomonas fluorescens
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.48
-
 30°C Pseudomonas fluorescens
10.02
-
 30°C Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate + H+
-
 Pseudomonas fluorescens acetate + CO2
-
 ?
malonate + H+
-
 Acinetobacter calcoaceticus acetate + CO2
-
 ?

Subunits

Subunits Comment Organism
multimer 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit Acinetobacter calcoaceticus
multimer 2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit Pseudomonas fluorescens