Literature summary for 4.1.1.86 extracted from

Nakao, H.; Ishii, M.; Shinoda, S.; Yamamoto, S.
Purification and some properties of a novel L-2,4-diaminobutyric acid decarboxylase from Vibrio alginolyticus (1989), J. Gen. Microbiol., 135, 345-351.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	L-2,4-diaminobutanoate induces enzyme expression	Vibrio alginolyticus

General Stability

General Stability	Organism
freezing and thawing of the purified enzyme causes almost complete loss of activity	Vibrio alginolyticus
pyridoxal 5'-phosphate is required for stability during storage	Vibrio alginolyticus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	over 90% inhibition at 1 mM	Vibrio alginolyticus
Ca2+	10% inhibition at 1 mM	Vibrio alginolyticus
Fe2+	over 90% inhibition at 1 mM	Vibrio alginolyticus
Hg2+	over 90% inhibition at 1 mM	Vibrio alginolyticus
additional information	the enzyme is not affected by GTP, ATP, DTT, Na+ or K+	Vibrio alginolyticus
propane-1,3-diamine	product inhibition, about 50% at 5 mM	Vibrio alginolyticus
Zn2+	over 90% inhibition at 1 mM	Vibrio alginolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0056	-	pyridoxal 5'-phosphate	pH 8.3, 37°C	Vibrio alginolyticus
0.081	-	L-2,4-diaminobutanoate	pH 8.3, 37°C	Vibrio alginolyticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	stimulates 30% at 20 mM	Vibrio alginolyticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
109000	-	4 * 109000, SDS-PAGE	Vibrio alginolyticus
450000	-	gel filtration	Vibrio alginolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-2,4-diaminobutanoate	Vibrio alginolyticus	propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine	propane-1,3-diamine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio alginolyticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
3660fold to homogeneity by ammonium sulfate fractionation, gel filtration, and two steps of ion exchange chromatography	Vibrio alginolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.21	-	purified enzyme	Vibrio alginolyticus

Storage Stability

Storage Stability	Organism
4°C, purified enzyme, pH 7.5, in presence of pyridoxal 5'-phosphate and NaN3, 3 weeks, stable	Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-2,4-diaminobutanoate	-	Vibrio alginolyticus	propane-1,3-diamine + CO2	-	?
L-2,4-diaminobutanoate	propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine	Vibrio alginolyticus	propane-1,3-diamine + CO2	-	?
additional information	no activity with 2,3-diaminopropionate, ornithine, lysine, or arginine	Vibrio alginolyticus	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 109000, SDS-PAGE	Vibrio alginolyticus

Synonyms

Synonyms	Comment	Organism
DABA decarboxylase	-	Vibrio alginolyticus
L-2,4-diaminobutyric acid decarboxylase	-	Vibrio alginolyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8.8	broad optimum	Vibrio alginolyticus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.8	10	about half maximal activity at pH 6.5 and pH 9.3	Vibrio alginolyticus

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	required, depletion leads to over 25% irreversible loss of activity	Vibrio alginolyticus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio alginolyticus	isoelectric focusing	-	6.8

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Release 2023.1 (January 2023)