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Literature summary for 4.1.1.86 extracted from

  • Nakao, H.; Takeuchi, K.; Shinoda, S.; Yamamoto, S.
    L-2,4,-Diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes (1990), FEMS Microbiol. Lett., 70, 61-66.
No PubMed abstract available
Search for more literature for 4.1.1.86

Activating Compound

Activating Compound Comment Organism Structure
DTT activates 8fold at 5-7 mM Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
carboxymethoxylamine PLP-dependent inhibitor, 42% inhibition at 0.5 mM, 65% inhibition at 1 mM Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 L-2,4-diaminobutanoate pH 8.3, 37°C Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 14fold at 20 mM Klebsiella aerogenes
Cu2+ activates 7fold at 20 mM Klebsiella aerogenes
Mg2+ required, activates 20fold at 20 mM, enhances activation by PLP Klebsiella aerogenes
Mn2+ activates 5fold at 20 mM Klebsiella aerogenes
Zn2+ activates 3fold at 20 mM Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2,4-diaminobutanoate Klebsiella aerogenes the enzyme is involved in polyamine biosynthesis propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate Klebsiella aerogenes NCTC 10006 the enzyme is involved in polyamine biosynthesis propane-1,3-diamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-
Klebsiella aerogenes NCTC 10006
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 360fold Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 partially purified enzyme Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2,4-diaminobutanoate
-
 Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate the enzyme is involved in polyamine biosynthesis Klebsiella aerogenes propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate
-
 Klebsiella aerogenes NCTC 10006 propane-1,3-diamine + CO2
-
 ?
L-2,4-diaminobutanoate the enzyme is involved in polyamine biosynthesis Klebsiella aerogenes NCTC 10006 propane-1,3-diamine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DABA-DC
-
 Klebsiella aerogenes
L-2,4-diaminobutyric acid decarboxylase
-
 Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
 Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
6 9 43% of maximal activity at pH 7.3, 60% at pH 9.0 Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate absolutely required for activity, highly activating in pesence of 20 mM Mg2+ Klebsiella aerogenes