Cloned (Comment) | Organism |
---|---|
into pET-28a vector containing an N-terminal His6-tag and expressed in Escherichia coli strain BL21 (DE3) cells | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
at 289 K using the hanging-drop vapor-diffusion technique. Apoenzyme obtained in two different space groups P212121 (without Mg2+) and I422 (in complex with Mg2+) and in the absence and presence of the product analog D-ribulose 5-phosphate. An 8 A alphaB-helix movement and other structural rearrangements around the active site between the apostructures and product analog bound structure. Conformational flexibility around the active site for substrate/product binding. Especially the alphaB-helix (residues 144-149) and the side chains of the conserved Arg144 and Arg197 exhibit openclosed conformational changes between the apo-structure and complex structure with ligand bound at the active site | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ dependent decarboxylation | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Streptococcus mutans UA159 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni2+-chelating affinity column and by gel filtration | Streptococcus mutans |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
3-keto-L-gulonate 6-phosphate decarboxylase | - |
Streptococcus mutans |
KGPDC | - |
Streptococcus mutans |