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Literature summary for 4.1.1.85 extracted from

  • Yew, W.S.; Gerlt, J.A.
    Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons (2002), J. Bacteriol., 184, 302-306.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
gene sgbH, expression in strain BL21(DE3) as N-terminally His10-tagged protein Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydro-L-gulonate 6-phosphate + H+ Escherichia coli K-12 SgaH/UlaD and SgbH L-xylulose 5-phosphate + CO2
-
 ?
additional information Escherichia coli K-12 enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
 gene ulaD or sgaH encoded in the yia-sga operon, and gene sgbH encoded in the yia-sgb operon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His10-tagged SgbH from strain BL21(DE3) to homogeneity, the His-tag is removed by thrombin Escherichia coli K-12

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydro-L-gulonate 6-phosphate + H+ SgaH/UlaD and SgbH Escherichia coli K-12 L-xylulose 5-phosphate + CO2
-
 ?
additional information enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview Escherichia coli K-12 ?
-
 ?
additional information enzymes SgaH/UlaD and SgbH are not active with 3-dehydro-L-gulonate Escherichia coli K-12 ?
-
 ?

Synonyms

Synonyms Comment Organism
3-keto-L-gulonate 6-phosphate decarboxylase
-
 Escherichia coli K-12
SgaH
-
 Escherichia coli K-12
SgbH
-
 Escherichia coli K-12
UlaD
-
 Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli K-12

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
 3-dehydro-L-gulonate 6-phosphate SgaH, pH 7.5, 25°C Escherichia coli K-12
64
-
 3-dehydro-L-gulonate 6-phosphate SgbH, pH 7.5, 25°C Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli K-12