Literature summary for 4.1.1.83 extracted from

Andrei, P.I.; Pierik, A.J.; Zauner, S.; Andrei-Selmer, L.C.; Selmer, T.
Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity (2004), Eur. J. Biochem., 271, 2225-2230.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloning of three genes encoding two subunits of the glycyl-radical enzyme and the activating enzyme, expressed in Escherichia coli	Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
460000	-	hetero-octameric catalytically competent complex, gel filtration	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	Q84F14	HpdA protein	-
Clostridioides difficile	Q84F15	HpdC protein	-
Clostridioides difficile	Q84F16	HpdB protein	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation of the small subunit is responsible for th change in oligomeric state from inactive homo-dimeric protein of Clostridium difficile to the recombinant hetero-octameric catalytically competent complex	Clostridioides difficile

Purification (Commentary)

Purification (Comment)	Organism
-	Clostridioides difficile

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(4-hydroxyphenyl)acetate + H+	-	Clostridioides difficile	4-methylphenol + CO2	-	?

Subunits

Subunits	Comment	Organism
dimer	beta2, decarboxylase purified from Clostridium difficile is an almost inactive homo-dimeric protein	Clostridioides difficile
octamer	beta4gamma4, small subunit HpdC has a molecular weight of 9598 Da as determined by MALDI TOF MS, the recombinant enzyme is a hetero-octameric catalytically competent complex	Clostridioides difficile

Synonyms

Synonyms	Comment	Organism
p-hydroxyphenylacetate decarboxylase	-	Clostridioides difficile

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Release 2023.1 (January 2023)