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Literature summary for 4.1.1.77 extracted from

  • Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K.
    Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain 10d: a different modified meta-cleavage pathway for 2-aminophenols (2006), Biosci. Biotechnol. Biochem., 70, 2653-2661.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 1 mM, 47% remaining activity Bordetella sp.
FeSO4 1 mM, 23% remaining activity Bordetella sp.
iodoacetic acid 1 mM, 64% remaining activity Bordetella sp.
K3Fe(CN)6 1 mM, 36% remaining activity Bordetella sp.
MnSO4 1 mM, 9% remaining activity Bordetella sp.
N-Methylmaleimide 1 mM, 58% remaining activity Bordetella sp.
p-chloromercuribenzoic acid 1 mM, 54% remaining activity Bordetella sp.
Tiron 1 mM, 59% remaining activity Bordetella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bordetella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
SDS-PAGE Bordetella sp.

Organism

Organism UniProt Comment Textmining
Bordetella sp.
-
-
-
Bordetella sp. 10d
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 cellulose column chromatography and DEAE-Cellulofine A800 column chromatography, DEAE-Toyopearl column chromatography, streptomycin sulfate precipitation, and ammonium sulfate precipitation Bordetella sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-amino-3-hydroxybenzoate-grown cell
-
Bordetella sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
cell extract Bordetella sp.
18
-
after 360fold purification Bordetella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Oxalocrotonate
-
Bordetella sp. 2-Oxopent-4-enoate + CO2
-
?
4-Oxalocrotonate
-
Bordetella sp. 10d 2-Oxopent-4-enoate + CO2
-
?

Synonyms

Synonyms Comment Organism
4OD
-
Bordetella sp.