Protein Variants | Comment | Organism |
---|---|---|
E109Q | 7fold increase in Km-value, 2270fold decrease in kcat, no influence of mutation to vinylpyruvate hydratase activity | Pseudomonas putida |
E111Q | Km-value comparable to wild-type, 3400fold decrease in kcat no influence of mutation to vinylpyruvate hydratase activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-2-fluorohex-2-en-4-ynoic acid | - |
Pseudomonas putida | |
(2E)-2-fluoropent-2-en-4-ynoic acid | - |
Pseudomonas putida | |
(2E)-2-fluoropenta-2,4-dienoic acid | - |
Pseudomonas putida | |
(2E,4E)-2-fluorohexa-2,4-dienedioic acid | - |
Pseudomonas putida | |
(2E,4E)-2-fluorohexa-2,4-dienoic acid | - |
Pseudomonas putida | |
(2Z)-2-fluorohex-2-en-4-ynedioic acid | - |
Pseudomonas putida | |
(2Z)-2-fluoropent-2-en-4-ynoic acid | - |
Pseudomonas putida | |
(2Z)-2-fluoropenta-2,4-dienoic acid | - |
Pseudomonas putida | |
(2Z,4E)-2-fluorohexa-2,4-dienoic acid | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
4-oxalocrotonate | mutant E111Q, in complex with vinylpuruvate hydratase | Pseudomonas putida | |
0.0066 | - |
4-oxalocrotonate | wild-type, in complex with vinylpuruvate hydratase | Pseudomonas putida | |
0.047 | - |
4-oxalocrotonate | mutant E109Q, in complex with vinylpuruvate hydratase | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Oxalocrotonate | - |
Pseudomonas putida | 2-Oxopent-4-enoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme forms a complex with vinylpyruvate hydratase, which is only active when stabilized in this complex | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-oxalocrotonate | mutant E111Q, in complex with vinylpuruvate hydratase | Pseudomonas putida | |
0.03 | - |
4-oxalocrotonate | mutant E109Q, in complex with vinylpuruvate hydratase | Pseudomonas putida | |
68 | - |
4-oxalocrotonate | wild-type, in complex with vinylpuruvate hydratase | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
(2E)-2-fluoropenta-2,4-dienoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.0003 | - |
(2Z)-2-fluorohex-2-en-4-ynedioic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.00034 | - |
(2Z)-2-fluoropenta-2,4-dienoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.0022 | - |
(2E,4E)-2-fluorohexa-2,4-dienoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.0068 | - |
(2E)-2-fluorohex-2-en-4-ynoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.0085 | - |
(2Z,4E)-2-fluorohexa-2,4-dienoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.014 | - |
(2E)-2-fluoropent-2-en-4-ynoic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.045 | - |
(2E,4E)-2-fluorohexa-2,4-dienedioic acid | pH 7.3, 22°C | Pseudomonas putida | |
0.05 | - |
(2Z)-2-fluoropent-2-en-4-ynoic acid | pH 7.3, 22°C | Pseudomonas putida |