KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
2-phenyl-2-methylmalonate | ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8) | Bordetella bronchiseptica | |
10.7 | - |
2-phenyl-2-methylmalonate | wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2) | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenyl-2-methylmalonate | - |
Bordetella bronchiseptica | (2R)-phenylpropionate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
316 | - |
2-phenyl-2-methylmalonate | wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2) | Bordetella bronchiseptica | |
358 | - |
2-phenyl-2-methylmalonate | ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8) | Bordetella bronchiseptica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor-independent enzyme | Bordetella bronchiseptica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.53 | - |
2-phenyl-2-methylmalonate | wild type enzyme, in MOPS buffer (5-10 mM, pH 7.2) | Bordetella bronchiseptica | |
36.16 | - |
2-phenyl-2-methylmalonate | ybbR-tagged AMDase, in Tris buffer (0.1 M, pH 8) | Bordetella bronchiseptica |