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Literature summary for 4.1.1.76 extracted from

  • Ijima, Y.; Matoishi, K.; Terao, Y.; Doi, N.; Yanagawa, H.; Ohta, H.
    Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism (2005), Chem. Commun. (Camb.), 2005, 877-879.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
C188S activity with methyl(2-thienyl)malonic acid is 0.7% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 2.8% of the wild-type activity, with formation of the opposite enantiomer Bordetella bronchiseptica
G74C activity with methyl(2-thienyl)malonic acid is 0.79% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.44% of the wild-type activity, with formation of the opposite enantiomer Bordetella bronchiseptica
G74C/C188S activity with methyl(2-thienyl)malonic acid is 0.42% of wild-type activity, activity with methyl(2-naphthyl)malonic acid is 0.13% of the wild-type activity, with formation of the opposite enantiomer Bordetella bronchiseptica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
 methyl(2-naphthyl)malonic acid wild-type enzyme Bordetella bronchiseptica
1.25
-
 methyl(2-naphthyl)malonic acid mutant enzyme G74C/C188S Bordetella bronchiseptica
1.26
-
 methyl(2-naphthyl)malonic acid mutant enzyme C188S Bordetella bronchiseptica
1.56
-
 methyl(2-naphthyl)malonic acid mutant enzyme G74C Bordetella bronchiseptica
5.03
-
 methyl(2-thienyl)malonic acid mutant enzyme G74C/C188S Bordetella bronchiseptica
6.24
-
 methyl(2-thienyl)malonic acid mutant enzyme G74C Bordetella bronchiseptica
12.5
-
 methyl(2-thienyl)malonic acid wild-type enzyme Bordetella bronchiseptica
14.5
-
 methyl(2-thienyl)malonic acid mutant enzyme C188S Bordetella bronchiseptica

Organism

Organism UniProt Comment Textmining
Bordetella bronchiseptica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl(2-naphthyl)malonic acid
-
 Bordetella bronchiseptica (2R)-2-(2-naphthyl)propanoic acid + CO2
-
 ?
methyl(2-thienyl)malonic acid
-
 Bordetella bronchiseptica (2S)-2-(2-thienyl)propanoic acid + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 methyl(2-naphthyl)malonic acid mutant enzyme G74C/C188S Bordetella bronchiseptica
0.34
-
 methyl(2-thienyl)malonic acid mutant enzyme G74C/C188S Bordetella bronchiseptica
0.5
-
 methyl(2-naphthyl)malonic acid mutant enzyme G74C Bordetella bronchiseptica
0.79
-
 methyl(2-thienyl)malonic acid mutant enzyme G74C Bordetella bronchiseptica
1.63
-
 methyl(2-thienyl)malonic acid mutant enzyme C188S Bordetella bronchiseptica
2.54
-
 methyl(2-naphthyl)malonic acid mutant enzyme C188S Bordetella bronchiseptica
31
-
 methyl(2-naphthyl)malonic acid wild-type enzyme Bordetella bronchiseptica
200
-
 methyl(2-thienyl)malonic acid wild-type enzyme Bordetella bronchiseptica