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Literature summary for 4.1.1.75 extracted from
- Intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the L-arginine catabolic pathway of Pseudomonas putida (1975), J. Biol. Chem., 250, 5322-5329.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-Hydroxy-delta-guanidino-N-pentanoate | weak | Pseudomonas putida |  |
| delta-Guanidinopentanoate | - |
Pseudomonas putida | |
| epsilon-Guanidinohexanoate | - |
Pseudomonas putida | |
| Ethylguanidine | - |
Pseudomonas putida | |
| gamma-Guanidinobutanoate | weak | Pseudomonas putida | |
| gamma-guanidinobutyraldehyde | - |
Pseudomonas putida | |
| Guanidinoacetate | weak | Pseudomonas putida | |
| Hexylguanidine | - |
Pseudomonas putida | |
| L-Arg | weak | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, half-maximal activity at 0.6 mM | Pseudomonas putida | |
| Co2+ | activates, half-maximal activity at 0.3 mM | Pseudomonas putida | |
| Mg2+ | activates, half-maximal activity at 0.3 mM | Pseudomonas putida | |
| Mn2+ | activates, half-maximal activity at 0.03 mM | Pseudomonas putida | |
| Ni2+ | activates | Pseudomonas putida | |
| Zn2+ | activates | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Guanidino-2-oxopentanoate | Pseudomonas putida | induced by Arg or gamma-guanidinobutyraldehyde | ? | - |
? | |
| 5-Guanidino-2-oxopentanoate | Pseudomonas putida P2 | induced by Arg or gamma-guanidinobutyraldehyde | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida P2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Guanidino-2-oxopentanoate | i.e. alpha-ketoarginine | Pseudomonas putida | 4-Guanidinobutyraldehyde + CO2 | - |
? | |
| 5-Guanidino-2-oxopentanoate | i.e. alpha-ketoarginine | Pseudomonas putida P2 | 4-Guanidinobutyraldehyde + CO2 | - |
? | |
| 5-Guanidino-2-oxopentanoate | induced by Arg or gamma-guanidinobutyraldehyde | Pseudomonas putida | ? | - |
? | |
| 5-Guanidino-2-oxopentanoate | induced by Arg or gamma-guanidinobutyraldehyde | Pseudomonas putida P2 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
30 min, stable | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 167 | - |
5-guanidino-2-oxopentanoate | - |
Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required | Pseudomonas putida | |
| thiamine diphosphate | required | Pseudomonas putida | |
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