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Literature summary for 4.1.1.73 extracted from

  • Furuyoshi, S.; Kawabata, N.; Tanaka, H.; Soda, K.
    Enzymic production of D-glycerate from L-tartrate (1989), Agric. Biol. Chem., 53, 2101-2105.
No PubMed abstract available
Search for more literature for 4.1.1.73

Application

Application Comment Organism
synthesis production of D-glycerate, which is a useful chiral synthon in synthetic organic chemistry Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong Pseudomonas sp.
EDTA 1 mM, at 30°C, complete inhibition, 1 mM MgCl2 or MnCl2 reactivates Pseudomonas sp.
Fe2+
-
 Pseudomonas sp.
L-Tartrate 1 mM/ml; strong substrate inhibition Pseudomonas sp.
Ni2+ weak Pseudomonas sp.
Zn2+ strong Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Pseudomonas sp.
Mn2+ activation Pseudomonas sp.
additional information not activated by Co2+ or Ca2+ Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tartrate Pseudomonas sp. inducible by L-tartrate D-glycerate + CO2
-
 ?
L-tartrate Pseudomonas sp. 5D1A inducible by L-tartrate D-glycerate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 soil isolate
-
Pseudomonas sp.
-
 group Ve-2
-
Pseudomonas sp. 5D1A
-
 group Ve-2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tartrate best substrate Pseudomonas sp. D-glycerate + CO2
-
 ?
L-tartrate direct conversion, with almost 100% selectivity Pseudomonas sp. D-glycerate + CO2
-
 ?
L-tartrate inducible by L-tartrate Pseudomonas sp. D-glycerate + CO2
-
 ?
L-tartrate best substrate Pseudomonas sp. 5D1A D-glycerate + CO2
-
 ?
L-tartrate direct conversion, with almost 100% selectivity Pseudomonas sp. 5D1A D-glycerate + CO2
-
 ?
L-tartrate inducible by L-tartrate Pseudomonas sp. 5D1A D-glycerate + CO2
-
 ?
meso-tartrate
-
 Pseudomonas sp. D-glycerate + CO2
-
 ?
additional information not: D-tartrate, DL-malate Pseudomonas sp. ?
-
 ?
additional information not: dihydroxyfumarate Pseudomonas sp. ?
-
 ?
additional information not: D-tartrate, DL-malate Pseudomonas sp. 5D1A ?
-
 ?
additional information not: dihydroxyfumarate Pseudomonas sp. 5D1A ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 optimum around and assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Pseudomonas sp.
7.5
-
 assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ requirement Pseudomonas sp.