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Literature summary for 4.1.1.72 extracted from
- Amino acids allosterically regulate the thiamine diphosphate-dependent alpha-keto acid decarboxylase from Mycobacterium tuberculosis (2008), J. Biol. Chem., 283, 5344-5354.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | stopped-flow kinetics show that MtKDC is allosterically activated by 2-oxoacids, also amino acids are potent activators | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta 2 (DE3) and various Escherichia coli strains | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | weak substrate inhibition | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
| 0.4 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
| 0.83 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
| 1.17 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
| 1.21 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
| 20.25 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
| 26.11 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
| 138.3 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59780 | - |
calculated from the deduced amino acid sequence | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WG37 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WG37 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange chromatography and gel filtration | Mycobacterium tuberculosis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
| (indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis H37Rv | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
| 2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | 2-methylbutanal + CO2 | - |
? | |
| 2-oxobutyrate | - |
Mycobacterium tuberculosis | propionaldehyde + CO2 | - |
? | |
| 2-oxohexanoate | - |
Mycobacterium tuberculosis | n-pentanal + CO2 | - |
? | |
| 2-oxoisohexanoate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
| 2-oxoisopentanoate | - |
Mycobacterium tuberculosis | 2-methylpropanal + CO2 | - |
? | |
| 2-oxopentanoate | - |
Mycobacterium tuberculosis | n-butanal + CO2 | - |
? | |
| 4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
| 4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
| benzoylformate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
| additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| Phenylpyruvate | - |
Mycobacterium tuberculosis | Phenylacetaldehyde + CO2 | - |
? | |
| Phenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | Phenylacetaldehyde + CO2 | - |
? | |
| pyruvate | - |
Mycobacterium tuberculosis | acetaldehyde + CO2 | - |
? | |
| pyruvate | - |
Mycobacterium tuberculosis H37Rv | acetaldehyde + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-keto acid decarboxylase | - |
Mycobacterium tuberculosis |
| MtKDC | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.14 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
| 2.98 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
| 3.89 | - |
4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | |
| 5.98 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis | |
| 6.95 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
| 7.06 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
| 7.5 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
| 18.57 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
| 19.75 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
| 28.35 | - |
2-oxoisopentanoate | - |
Mycobacterium tuberculosis | |
| 31.5 | - |
2-Oxoisohexanoate | - |
Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 124 | - |
pyruvate | weak substrate inhibition is detected for pyruvate | Mycobacterium tuberculosis | |
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