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Literature summary for 4.1.1.72 extracted from

  • Chuang, J.L.; Wynn, R.M.; Song, J.L.; Chuang, D.T.
    GroEL/GroES-dependent reconstitution of a2b2 tetramers of human mitochondrial branched chain alpha-ketoacid decarboxylase. Obligatory interaction of chaperonins with an alphabeta dimeric intermediate (1999), J. Biol. Chem., 274, 10395-10404.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
2 * 45500, alpha subunit, + 2 * 37800, beta subunit, SDS-PAGE, enzyme forms part of the branched chain alpha-ketoacid dehydrogenase complex Homo sapiens
45500
-
2 * 45500, alpha subunit, + 2 * 37800, beta subunit, SDS-PAGE, enzyme forms part of the branched chain alpha-ketoacid dehydrogenase complex Homo sapiens
171000
-
with His6-tagged alpha subunits, gel filtration, sucrose density gradient Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
after denaturation in 8 M urea, reconstitution at 23°C with absolute requirement of chaperonins GroEL/GroES and MgATP2- Homo sapiens

Subunits

Subunits Comment Organism
tetramer 2 * 45500, alpha subunit, + 2 * 37800, beta subunit, SDS-PAGE, enzyme forms part of the branched chain alpha-ketoacid dehydrogenase complex Homo sapiens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required Homo sapiens